Report of the committee on human gene mapping by recombinant DNA techniques.
Report of the committee on human gene mapping by recombinant DNA techniques.
复制标题
通过重组 DNA 技术进行人类基因作图委员会的报告。
DOI：
10.1159/000132011
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
J. Mandel
中科院分区：
文献类型：
--
作者：
H. Willard;M. Skolnick;P. Pearson;J. Mandel
这是该委员会向人类基因图谱研讨会提交的第二份报告。由于重组DNA研究的迅速进展，人们认为，关于重组DNA的统一报告将继续是有用的。这份报告比第一份报告要大得多，也更复杂，如果该领域继续以目前的速度扩大，该委员会的改组可能是必要的。表I是1981年6月在HGM 6和1983年8月在该会议上报道的克隆基因、限制性片段长度多态性（RFLP）和任意DNA片段的数量的比较。该表显示了每类克隆的数量大约增加了6倍，克隆的DNA片段总数从57个DNA片段增加到364个DNA片段。我们试图收集有关克隆基因和基因定位所需的任意DNA片段的信息。为了实现这一点，报告克隆基因的表格是从耶鲁基因图谱项目报告基因的更通用表格中衍生出来的。该表是在1983年1月由Ray白色和LL Cavalli-Sforza在迈阿密的霍华德休斯研究所组织的RFLP研讨会上开发的，在那里对已知的RFLP列表进行了更新。这个表格在今年夏天分发给了一大批研究人员，以增加我们对克隆基因和DNA片段的覆盖面。本表副本作为本报告的附录，可用于报告更多克隆基因和多态性。
This is the second report of this committee to the Human Gene Mapping Workshop. Because of the rapid progress in recombinant DNA research it was felt that a unified report on Recombinant DNA would continue to be useful. This report is substantially larger and more complex than the first report, and if the fie ld contiues to expand at its current rate, a reorganization of this committee could be essential. Table I is a comparison of the number of cloned genes, restriction fragment length polymorphisms (RFLPs), and arbitrary DNA fragments reported in June, 1981, at HGM 6, and August, 1983 at this conference. This table shows approximately a six-fold increase in the number of clones in each category, with the total number of cloned DNAs increasing from 57 DNA segments to 364 DNA segments.We have attempted to compile information on cloned genes and arbitrary DNA segments of interest to gene mapping. To accomplish this, a form for reporting cloned genes was derived from the more general form for reporting genes to the Yale Gene Mapping project. The form was developed at an RFLP workshop organized by Ray White and LL Cavalli-Sforza, held at the Howard Hughes Institute in Miami in January, 1983, where an update to the list of known RFLPs was compiled. This form was distributed to a large set of investigators this summer to increase our coverage of cloned genes and DNA segments. A copy of this form appears as an appendix to this report so that it can be used for reporting further cloned genes and polymorphisms.