Clinal variation in the non-acclimated and cold-acclimated freezing tolerance of Arabidopsis thaliana accessions.
Clinal variation in the non-acclimated and cold-acclimated freezing tolerance of Arabidopsis thaliana accessions.
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DOI：
10.1111/j.1365-3040.2012.02522.x
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发表时间：
2012-10
期刊：
影响因子：
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通讯作者：
E. Zuther;Elisa Schulz;L. Childs;D. Hincha
中科院分区：
文献类型：
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作者：
E. Zuther;Elisa Schulz;L. Childs;D. Hincha
拟南芥是一个地理分布广泛的物种，由遗传和表型不同的地方种质组成。这些差异可能构成了环境适应和纬度渐变的抗冻性已被证明。许多植物，包括拟南芥，在冷暴露（冷适应）后表现出更强的抗冻性。在这里，我们提出的证据，地理倾斜（纬度和纵向）在驯化（ACC）和非驯化（NA）的抗冻性，估计从54个加入电解质渗漏测量。脯氨酸含量与抗寒性无相关性，糖（Glc、Fru、Suc、Raf）含量处于ACC状态，而不处于NA状态。采用定量RT-PCR方法检测了冷适应前后14个冷诱导基因的表达水平。CBF 1、CBF 2和CBF 3基因的表达与抗寒性无关。然而，一些CBF调节（COR）基因的表达与ACC的抗冻性特异相关。仅在非适应条件下观察到CBF和COR基因表达之间的紧密相关性，其中CBF和COR表达还与生物钟的组成部分PRR 5的表达相关。总的来说，这项研究揭示了植物抗冻性和冷驯化及其地理依赖性的分子决定因素。
Arabidopsis thaliana is a geographically widely spread species consisting of local accessions differing both genetically and phenotypically. These differences may constitute environmental adaptations and a latitudinal cline in freezing tolerance has been shown previously. Many plants, including Arabidopsis, exhibit increased freezing tolerance after cold exposure (cold acclimation). Here we present evidence for geographical clines (both latitudinal and longitudinal) in acclimated (ACC) and non-acclimated (NA) freezing tolerance, estimated from electrolyte leakage measurements on 54 accessions. Leaf Pro contents were not correlated with freezing tolerance, while sugar contents (Glc, Fru, Suc, Raf) were in the ACC, but not the NA state. Expression levels of 14 cold-induced genes were investigated before and after 2 weeks of cold acclimation by quantitative RT-PCR. Expression of the CBF1, 2 and 3 genes was not correlated with freezing tolerance. The expression of some CBF-regulated (COR) genes, however, was correlated specifically with ACC freezing tolerance. A tight correlation between CBF and COR gene expression was only observed under non-acclimating conditions, where CBF and COR expression were also correlated with the expression of PRR5, a component of the circadian clock. Collectively, this study sheds new light on the molecular determinants of plant-freezing tolerance and cold acclimation and their geographical dependence.