Centerband-only analysis of rotor-unsynchronized spin-echo for measurement of lipid 31P chemical shift anisotropy.
Centerband-only analysis of rotor-unsynchronized spin-echo for measurement of lipid 31P chemical shift anisotropy.
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转子不同步自旋回波的仅中心带分析，用于测量脂质 31P 化学位移各向异性。
DOI：
10.1002/mrc.4247
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Shigeru Matsuoka*
中科院分区：
文献类型：
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作者：
Yuichi Umegawa;Toshiyuki Yamaguchi;Michio Murata;Shigeru Matsuoka*
磷脂的结构多样性和分子柔性对于生物膜在许多细胞过程中发挥关键作用是必不可少的。揭示单个脂质在膜动力学中的行为对于理解细胞膜生物功能的分子机制至关重要。本文介绍了一种通过测量魔角旋转（MAS）条件下31 P化学位移各向异性（CSA）来研究多组分系统中脂质分子动力学的简单方法。为了实现信号分离和CSA确定，我们利用了转子非同步自旋回波（COARSE）的仅中心带分析。该分析基于转子非同步自旋回波实验中中心带强度沿着脉冲间延迟时间的周期性调制的曲线拟合。通过使用磷脂囊泡、三组分脂筏模型系统和古菌紫色膜来检查COARSE的效用。我们发现，这种方法的明显优势是高分辨率和高灵敏度，这是由适度的MAS速度和具有短自旋回波延迟的一维采集提供的。COARSE为CSA测量提供了一种替代方法，该方法在脂质多态性的研究中是有效的。版权所有© 2015约翰威利父子有限公司.
Structural diversity and molecular flexibility of phospholipids are essential for biological membranes to play key roles in numerous cellular processes. Uncovering the behavior of individual lipids in membrane dynamics is crucial for understanding the molecular mechanisms underlying biological functions of cell membranes. In this paper, we introduce a simple method to investigate dynamics of lipid molecules in multi‐component systems by measuring the31P chemical shift anisotropy (CSA) under magic angle spinning (MAS) conditions. For achieving both signal separation and CSA determination, we utilized a centerband‐only analysis of rotor‐unsynchronized spin echo (COARSE). This analysis is based on the curve fitting of periodic modulation of centerband intensity along the interpulse delay time in rotor‐unsynchronized spin‐echo experiments. The utility of COARSE was examined by using phospholipid vesicles, a three‐component lipid raft model system, and archaeal purple membranes. We found that the apparent advantages of this method are high resolution and high sensitivity given by the moderate MAS speed and the one‐dimensional acquisition with short spin‐echo delays. COARSE provides an alternative method for CSA measurement that is effective in the investigation of lipid polymorphologies. Copyright © 2015 John Wiley & Sons, Ltd.