MicroRNA-204 suppressed proliferation and motility capacity of human hepatocellular carcinoma via directly targeting zinc finger E-box binding homeobox 2
MicroRNA-204 suppressed proliferation and motility capacity of human hepatocellular carcinoma via directly targeting zinc finger E-box binding homeobox 2
复制标题
MicroRNA-204通过直接靶向锌指E-box结合同源框2抑制人肝细胞癌的增殖和运动能力
DOI：
10.3892/ol.2017.5907
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发表时间：
2017-05-01
期刊：
影响因子：
2.9
通讯作者：
Lin, Qin
中科院分区：
文献类型：
--
作者：
Hu, Bin;Sun, Ming;Lin, Qin
MicroRNA-204(miR-204)的异常表达水平在各种类型的人类癌症中被发现。然而，miR-204的表达和功能以及参与肝细胞癌发生和发展的潜在分子机制仍需进一步研究。本研究结果表明miR-204在肝细胞癌组织和细胞系中表达下调。值得注意的是，锌指E盒结合同源框2(ZEB2)被确定为miR-204在肝癌中的直接靶点。此外，miR-204在转录后水平负向调节肝癌细胞中ZEB2的表达水平。在功能研究中，miR-204的过表达抑制了肝癌细胞的增殖、迁移和侵袭。此外，ZEB2基因的敲除可能模拟了miR-204在肝癌细胞中的功能，提示ZEB2是miR-204的直接功能靶点。综上所述，本研究结果表明miR-204通过直接靶向ZEB2抑制肝癌细胞的生长、迁移和侵袭，有望成为治疗肝癌的新靶点。
Abnormal expression levels of microRNA-204 (miR-204) have been identified in various types of human cancer. However, the expression and functions of miR-204, and the underlying molecular mechanism involved in the initiation and progression of hepatocellular carcinoma (HCC), require further investigation. The results of the present study demonstrated that miR-204 is downregulated in HCC tissues and cell lines. Notably, zinc finger E-box binding homeobox 2 (ZEB2) was identified as a direct target of miR-204 in HCC. In addition, miR-204 negatively regulates ZEB2 expression level in HCC cells at the post-transcriptional level. In functional studies, the overexpression of miR-204 inhibited the proliferation, migration and invasion of HCC cells. Furthermore, the knockdown of ZEB2 may mimic the functions of miR-204 in HCC cells, suggesting that ZEB2 is a direct functional target of miR-204. In conclusion, the results of the present study indicated that miR-204 suppresses the tumor growth, migration and invasion of HCC cells by directly targeting ZEB2, and may serve as a novel therapeutic target for HCC.