Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus
Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus
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DOI：
10.1016/j.aquaculture.2020.735493
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发表时间：
2020-11
期刊：
影响因子：
4.5
通讯作者：
Shaoshuai Liang;Wenxiang Wang;Lijuan Wang;Zhihao Wu;Y. Zou;X. Tan;Yan Liu;Zhuangzhuang Peng;F. You
中科院分区：
文献类型：
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作者：
Shaoshuai Liang;Wenxiang Wang;Lijuan Wang;Zhihao Wu;Y. Zou;X. Tan;Yan Liu;Zhuangzhuang Peng;F. You
生殖系α因子（一种碱性螺旋-环-螺旋转录因子，FIGLA）基因在雌性生殖细胞中特异性表达，对脊椎动物的卵巢分化和发育至关重要。然而，关于其在鱼类性腺分化和发育中的作用的研究还很有限。本研究从中国、日本和韩国的重要海水养殖鱼类橄榄比目鱼中克隆并鉴定了afiglagene。该基因全长沿着为1316 bp，开放阅读框为609 bp。实时定量聚合酶链反应（qPCR）分析表明，它在卵巢和雌性大脑中大量表达。在卵巢发育的I-V期，Po-figlamRNA的表达水平在II期达到高峰，之后逐渐下降，而Po-figlamRNA则随着睾丸发育沿着有少量表达。单色和双色原位杂交结果表明，该基因主要定位于卵母细胞中。利用RNAi技术敲低卵巢中的Po-figlain基因，可导致其表达下调，卵母细胞数量减少。此外，qPCR结果还显示了Po-figladin在性腺分化期的差异表达模式。在外源性17β-雌二醇（5 ppm）处理组中，Po-figla在分化和分化的卵巢中表达水平组成性增加，而在睾酮（5 ppm）处理组中，在睾丸分化期，Po-figla在睾丸中表达较低。我们的研究结果为Po-figla参与P.卵巢的分化和发育，尤其是在鱼类卵母细胞的增殖中可能起作用。这些结果为鱼类繁殖和性腺发育调控提供了基础数据。
Factor in the germ line alpha (a basic helix-loop-helix transcription factor, FIGLA) gene specifically expresses in female germ cells and is essential for ovarian differentiation and development in vertebrates. However, research on the roles in fish gonadal differentiation and development is limited. In this study, we cloned and characterized afiglagene from the olive flounder,Paralichthys olivaceus, an important marine cultured fish in China, Japan, and Korea. The full-length cDNA of thePo-figlais 1316 bp along with a 609 bp open reading frame. Real-time quantitative polymerase chain reaction analysis (qPCR) revealed that it was abundantly expressed in the ovary and female brain. During stages I-V of the ovary, the level ofPo-figlamRNA reached the peak at stage II, and then gradually decreased, whilePo-figlamRNA was slightly expressed along with the testicular development. Further, one color and two-colorin situhybridization results showed that the gene was mainly located in oocytes. Knocking downPo-figlain the ovary using RNAi led to its down-regulated expression and the reduction of oocyte number. In addition, qPCR results presented the differential expression patterns ofPo-figladuring gonadal differentiation period. In the exogenous 17β-estradiol treatment group (5 ppm),Po-figlaexpression levels constitutively increased in the differentiating and differentiated ovaries, while in the testosterone (5 ppm) treatment group, it was lower expressed in the testis during testicular differentiation period. Our findings provide evidence thatPo-figlais involved inP. olivaceusovarian differentiation and development, especially might have roles in the proliferation of fish oocytes. These findings provide the basic data for fish reproduction and gonadal development regulation in aquaculture.