Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
Stable and biocompatible hydrogel composites based on collagen and dialdehyde carboxymethyl cellulose in a biphasic solvent system.
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DOI：
10.1016/j.carbpol.2019.114974
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发表时间：
2019-10
影响因子：
11.2
通讯作者：
Huan Yang;Lirui Shen;Honghong Bu;Guoying Li
中科院分区：
文献类型：
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作者：
Huan Yang;Lirui Shen;Honghong Bu;Guoying Li
稳定的水凝胶具有机械强度强的基质微环境，是三维细胞培养的良好生物材料。酸性胶原蛋白溶液通常与化学交联剂相结合，以快速形成网络。因此，双醛羧甲基纤维素(DCMC)因其良好的生物相容性和适宜的化学反应活性而被选为最佳交联剂。利用一种新型的双相醋酸/1-乙基-3-甲基咪唑乙酸酯(AA/[EMIM][Ac])溶剂体系，实现了屏蔽两种相反电荷生物材料之间的静电吸引和获得浓缩的胶原蛋白溶液。随着胶原浓度的增加(5~25 mg/mL)，水凝胶复合材料的力学性能、热变性温度、抗酶能力和致密结构都得到了改善。细胞增殖实验表明，所制得的DCMC-胶原水凝胶复合材料具有良好的生物相容性。这项研究为在两相溶剂体系中将浓胶原蛋白溶液与DCMC共混来制备稳定的生物相容性水凝胶复合材料提供了一种很有前途的策略。
Stable hydrogels with a mechanically strong matrix microenvironment are favorable biomaterials for three-dimensional cell culture. Acidic collagen solution is commonly combined with chemical crosslinkers for rapid network formation. Herein, dialdehyde carboxymethyl cellulose (DCMC) was selected as an optimal crosslinking reagent for its excellent biocompatibility and suitable chemical reactivity. Both shielding of electrostatic attractions between these two oppositely charged biomaterials and obtaining concentrated collagen solution were achieved using a novel biphasic acetic acid /1-ethyl-3-methylimidazolim acetate (AA/[EMIM][Ac]) solvent system. Hydrogel composites containing more crosslinks were obtained by increasing collagen concentrations (5–25 mg/mL), as confirmed by the improved mechanical properties, thermal denaturation temperature, anti-enzymatic ability and compact microstructure. Moreover, cell proliferation assay demonstrated that all the obtained DCMC-crosslinked collagen hydrogel composites ensures commendable biocompatibility. This study provides a promising strategy for manipulating stable and biocompatible hydrogel composites by blending concentrated collagen solution with DCMC in a biphasic solvent system.