Nicotinic infertility: assessing DNA and plasma membrane integrity of human spermatozoa
Nicotinic infertility: assessing DNA and plasma membrane integrity of human spermatozoa
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DOI：
10.1111/j.1439-0272.2004.00623.x
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发表时间：
2004-10-01
期刊：
影响因子：
2.4
通讯作者：
Arabi, M
中科院分区：
文献类型：
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作者：
Arabi, M
不孕症仍然是社会的一个主要问题，最近的数据表明，四对夫妇中就有一对存在不孕症。本研究的目的是评价尼古丁（0.25、0.5和0.75 mM）作为香烟烟雾的主要成分在体外对精子膜[通过精子压积和脂质过氧化（LPO）试验]、DNA完整性（通过彗星试验）和精子活力（通过伊红染色）的影响。精子样本用磷酸盐缓冲盐水洗涤和稀释。添加尼古丁（主要浓度为0.75 mM）后，观察到精子压积值下降和硫代巴比妥酸反应物质/LPO速率增加，表明尼古丁对精子膜完整性具有有害影响。LPO率与精子活率呈显著负相关（r = -0.990）。彗星试验结果显示，尼古丁可引起精子核双链DNA断裂（0.75 mM浓度时断裂率为11%）。发现LPO率和彗星百分比之间的r值为+0.976。总之，尼古丁被证明是一种潜在的氧化剂类的环境因素的精子质膜和DNA的完整性。
Infertility remains a major problem in society, with recent data suggesting its presence in one of four couples. The objective of the present study was to evaluate the impact of nicotine (0.25, 0.5 and 0.75 mM), as a major component of cigarette smoke, in vitro, on sperm membrane [by spermatocrit and lipoperoxidation (LPO) tests], DNA integrity (by Comet assay), and viability of spermatozoa (by eosin staining) from normozoospermic men. Sperm samples were washed and diluted with phosphate-buffered saline. A drop in spermatocrit values and an increase in thiobarbituric acid-reactive substances/LPO rate was observed with the addition of nicotine, predominantly at a concentration of 0.75 mM, indicating a deleterious effect of nicotine on sperm membrane intactness. There was also a strong negative correlation between LPO rate and percentage viable sperm cell (r = -0.990). Data obtained from Comet assay technique revealed that nicotine could induce double-stranded DNA breaks (11% in 0.75 mM concentration) in the sperm nuclei. The value of r between LPO rate and percentage Comets was found to be +0.976. Taken together, nicotine proved to be a potential oxidant agent in the category of environmental factors to the integrity of sperm plasma membrane and DNA.