Very rapid decay of mature B lymphocytes in the spleen.
Very rapid decay of mature B lymphocytes in the spleen.
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DOI：
10.1084/jem.154.3.994
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发表时间：
1981-09-01
期刊：
影响因子：
--
通讯作者：
Coutinho A
中科院分区：
文献类型：
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作者：
de Freitas AA;Coutinho A
为了确定免疫活性B淋巴细胞在外周的持久性，无论细胞分裂，我们遵循了脂多糖（LPS）反应性B细胞在LPS无应答，组织相容性主机的衰减。通过对各种细胞群进行有限稀释分析，可以精确地确定转移细胞的数量和在受体中持续存在的细胞的数量。每天50%的衰减率进行了测定。因为我们可以排除供体细胞的免疫消除，并且转移细胞的数量太低而不会导致生理周转率的总体改变，所以我们得出结论，大多数LPS反应性B淋巴细胞和整个B细胞区室的大部分显示出这种惊人的高衰减率。这里介绍的方法可能被证明在各种克隆测定中是有用的，因为它可以检测以非常低的频率存在的细胞。
To determine the persistence of immunocompetent B lymphocytes at the periphery, regardless of cell division, we have followed the decay of lipopolysaccharide (LPS)-reactive B cells in LPS-nonresponder, histocompatible hosts. Both the numbers of transferred cells and of those persisting in the recipients could be determine with precision by limiting dilution analysis of the various cell populations. Decay rates of 50% per day were determined. Because we can exclude immune elimination of donor cells and the numbers of transferred cells were too low to result in gross alterations of the physiologic turnover rates, we conclude that the majority of LPS-reactive B lymphocytes and a large part of the whole B cell compartment show this astonishingly high rate of decay. The method introduced here might prove useful in a variety of clonal assays, as it can detect cells present at a very low frequency.