Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition
Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition
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DOI：
10.1016/s0142-9612(03)00052-8
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发表时间：
2003-06-01
期刊：
影响因子：
14
通讯作者：
Bhatia, S
中科院分区：
文献类型：
--
作者：
Vozzi, G;Flaim, C;Bhatia, S
用于组织工程的可生物降解的三维支架的构建先前已经描述了使用各种模制和快速原型技术。在这项研究中，我们报告和比较两种方法制作聚（DL-丙交酯-共-乙交酯）（PLGA）支架的特征尺寸约为10-30妈妈。第一种技术，压力辅助微量注射器，是基于使用的微量注射器，利用计算机控制的，三轴微定位器，它允许控制电机的速度和位置。通过施加20-300 mm Hg的恒定压力从注射器的针头沉积PLGA溶液，导致受控的聚合物沉积。第二种技术是基于“软光刻”的方法，利用聚（二甲基硅氧烷）模具。第二种技术的三种变化：聚合物浇铸，微流体灌注，和旋涂。聚合物浓度，溶剂组合物，和模具尺寸的影响，通过光学和电子显微镜评价所得的支架。作为组织工程应用中支架效用的概念验证，通过热层压形成多层结构，并通过颗粒浸出使支架多孔。这些简单的方法，形成PLGA支架与微尺度的功能，可以作为有用的工具，探索组织工程中的结构/功能关系。(C)2003爱思唯尔科技有限公司版权所有。
Construction of biodegradable, three-dimensional scaffolds for tissue engineering has been previously described using a variety of molding and rapid prototyping techniques. In this study, we report and compare two methods for fabricating poly(DL-lactide-co-glycolide) (PLGA) scaffolds with feature sizes of approximately 10-30 mum. The first technique, the pressure assisted microsyringe, is based on the use of a microsyringe that utilizes a computer-controlled, three-axis micropositioner, which allows the control of motor speeds and position. A PLGA solution is deposited from the needle of a syringe by the application of a constant pressure of 20-300 mm Hg, resulting in a controlled polymer deposition. The second technique is based on 'soft lithographic' approaches that utilize a poly(dimethylsiloxane) mold. Three variations of the second technique are presented: polymer casting, microfluidic perfusion, and spin coating. Polymer concentration, solvent composition, and mold dimensions influenced the resulting scaffolds as evaluated by light and electron microscopy. As a proof-of-concept for scaffold utility in tissue engineering applications, multilayer structures were formed by thermal lamination, and scaffolds were rendered porous by particulate leaching. These simple methods for forming PLGA scaffolds with microscale features may serve as useful tools to explore structure/function relationships in tissue engineering. (C) 2003 Elsevier Science Ltd. All rights reserved.