SUCL+ ENCODES A PREDICTED 13-KILODALTON PROTEIN THAT IS ESSENTIAL FOR CELL VIABILITY AND IS DIRECTLY INVOLVED IN THE DIVISION CYCLE OF SCHIZOSACCHAROMYCES-POMBE
SUCL+ ENCODES A PREDICTED 13-KILODALTON PROTEIN THAT IS ESSENTIAL FOR CELL VIABILITY AND IS DIRECTLY INVOLVED IN THE DIVISION CYCLE OF SCHIZOSACCHAROMYCES-POMBE
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DOI：
10.1128/mcb.7.1.504
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发表时间：
1987-01-01
影响因子：
5.3
通讯作者：
BEACH, D
中科院分区：
文献类型：
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作者：
HINDLEY, J;PHEAR, G;BEACH, D
Suc 1+最初被鉴定为DNA序列，其在高拷贝数下拯救了携带cdc 2细胞周期控制基因的某些温度敏感等位基因的粟酒裂殖酵母菌株。我们确定了1,083个碱基对的Suc 1 + DNA片段的核苷酸序列，并绘制了S1的866个核苷酸的RNA转录本。该基因的蛋白质编码序列被115和51个碱基对的两个插入序列中断。该基因的预测翻译产物是13千道尔顿的蛋白质。在二倍体S.粟酒属菌株。携带该基因无效等位基因的萌发孢子能够进行非常有限的细胞分裂，随后许多细胞变得高度伸长。Suc 1+基因在异源S.粟酒启动子Suc 1+的过表达不是致命的，但会导致分裂延迟，使得细胞在分裂时大约是正常长度的两倍。这些数据表明Suc 1+编码一种在S细胞分裂周期中发挥直接作用的蛋白质。粟酒
Suc1+ was originally identified as a DNA sequence that, at high copy number, rescued Schizosaccharomyces pombe strains carrying certain temperature-sensitive alleles of the cdc2 cell cycle control gene. We determined the nucleotide sequence of a 1,083-base-pair Suc1+ DNA fragment and S1 mapped its 866-nucleotide RNA transcript. The protein-coding sequence of the gene is interrupted by two intervening sequences of 115 and 51 base pairs. The predicted translational product of the gene is a protein of 13 kilodaltons. A chromosomal gene disruption of Suc1+ was constructed in a diploid S. pombe strain. Germinating spores carrying a null allele of the gene were capable of very limited cell division, following which many cells became highly elongated. The Suc1+ gene was also strongly overexpressed under the control of a heterologous S. pombe promoter. Overexpression of Suc1+ is not lethal but causes a division delay such that cells are approximately twice the normal length at division. These data suggest that Suc1+ encodes a protein which plays a direct role in the cell division cycle of S. pombe.