Establishment and characterization of acute B-cell lymphocytic leukemia cell line showing (8;14) and (14;18) chromosome translocation.
Establishment and characterization of acute B-cell lymphocytic leukemia cell line showing (8;14) and (14;18) chromosome translocation.
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显示 (8;14) 和 (14;18) 染色体易位的急性 B 细胞淋巴细胞白血病细胞系的建立和表征。
DOI：
10.1159/000205054
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发表时间：
1990
影响因子：
2.4
通讯作者：
K. Takatsuki
中科院分区：
文献类型：
--
作者：
H. Matsuzaki;H. Hata;N. Asou;H. Suzushima;Y. Akahoshi;M. Yoshida;S. Nagakura;T. Ishii;I. Sanada;K. Takatsuki
表达两种癌基因产物c-myc和bcl-2的kkm - 2b细胞系从急性淋巴细胞白血病患者中建立。14和14；18染色体易位。细胞系表面标记研究显示，细胞HLA-DR、CALLA （CD10）、B1 （CD20）和B4 （CD19）阳性，T11 （CD2）阴性。患者外周血新鲜细胞无表面免疫球蛋白，而kkm - 2b细胞呈mu阳性。λ型表面免疫球蛋白。细胞系的细胞遗传学分析显示了两个易位，t(8;14) （q24;q32）和t(14;18)（q32;q21）。kkm - 2b DNA的Southern blot分析检测到c-myc和bcl-2基因重排。Northern blot分析显示c-myc和bcl-2 mrna的产生。这些结果表明，两种癌基因通过两种免疫球蛋白基因的易位而被激活。
Cell line KHM-2B expressing two oncogene products, c-myc and bcl-2, was established from a patient with acute lymphocytic leukemia with an 8;14 and 14;18 chromosome translocation. Surface marker studies of the cell line showed that the cells were positive for HLA-DR, CALLA (CD10), B1 (CD20) and B4 (CD19), but negative for T11 (CD2). The fresh cells from peripheral blood of the patient had no surface immunoglobulins, whereas KHM-2B cells were positive for mu.lambda type surface immunoglobulin. A cytogenetic analysis of the cell line revealed two translocations, t (8;14) (q24;q32) and t(14;18)(q32;q21). Rearrangement of the c-myc and bcl-2 genes was detected by Southern blot analysis of the KHM-2B DNA. Northern blot analysis revealed production of c-myc and bcl-2 mRNAs. These results indicated that two oncogenes were activated by two translocations to immunoglobulin genes.