INCREASED SYNTHESIS OF A LOW-MOLECULAR WEIGHT PROTEIN IN VINCRISTINE-RESISTANT CELLS
INCREASED SYNTHESIS OF A LOW-MOLECULAR WEIGHT PROTEIN IN VINCRISTINE-RESISTANT CELLS
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DOI：
10.1016/0006-291x(81)91736-8
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
BIEDLER, JL
中科院分区：
文献类型：
--
作者：
MEYERS, MB;BIEDLER, JL
在长春新碱耐药的中国仓鼠细胞(DC-3F/VCRd-5)中，用双向凝胶电泳法鉴定了一种19,000道尔顿多肽(等电点为5.7)。在DC-3F/VCRd-5的一个突变株中，该蛋白的量减少，而在对照DC-3F细胞中只检测到微量的该蛋白。在长春新碱耐药小鼠中也发现了类似的蛋白质(Mr[分子比]=19,000；PI=5.7)。两个对长春新碱耐药的人神经母细胞瘤细胞系同样含有高水平的低分子酸性蛋白。DC-3F/VCRd-5细胞中19,000道尔顿多肽的生物合成增加与中期染色体上存在均一染色区HSR相一致。
A 19,000-dalton peptide (pI = 5.7) that is synthesized in increased amounts in vincristine-resistant Chinese hamster cells (DC-3F/VCRd-5) has been identified by two-dimensional gel electrophoresis. Reduced amounts of the protein were present in a revertant line of DC-3F/VCRd-5, and only trace amounts were detected in control DC-3F cells. A similar protein (Mr [molecular ratio] = 19,000; PI = 5.7) was also found in a vincristine-resistant mouse line. Two vincristine-resistant human neuroblastoma cell lines likewise contained elevated levels of a low molecular weight acidic protien. Increased biosynthesis of the 19,000-dalton polypeptide in DC-3F/VCRd-5 cells coincides with the presence of a homogeneously staining region, HSR, on a metaphase chromosome.