Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions
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DOI：
10.3390/ijms21020486
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发表时间：
2020-01
影响因子：
5.6
通讯作者：
Zhuoheng Zhong;T. Furuya;Kimitaka Ueno;H. Yamaguchi;Keisuke Hitachi;K. Tsuchida;M. Tani;Jingkui Tian;S. Komatsu
中科院分区：
文献类型：
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作者：
Zhuoheng Zhong;T. Furuya;Kimitaka Ueno;H. Yamaguchi;Keisuke Hitachi;K. Tsuchida;M. Tani;Jingkui Tian;S. Komatsu
提高大豆的生长和对环境胁迫的耐受性是可持续发展的关键。毫米波是一个射频波段，波长范围为1-10毫米，对生物体有动态影响。为了研究毫米波辐射对大豆幼苗的潜在影响，进行了形态学和蛋白质组学分析。毫米波照射提高了大豆根/下胚轴的生长和对水淹胁迫的耐受性。蛋白质组学分析表明，辐射大豆幼苗在生长过程中的氧化胁迫下恢复，而糖酵解和抗坏血酸/谷胱甘肽代谢相关的蛋白质没有受到影响。免疫印迹分析证实了毫米波对糖酵解和氧化还原相关途径的促进作用。淹水胁迫下，未辐照大豆幼苗的糖代谢受到抑制，而辐照大豆幼苗的糖代谢被激活，尤其是海藻糖的合成。这些结果表明，毫米波辐射促进大豆幼苗在氧化胁迫下的恢复，通过调节糖酵解和氧化还原相关途径对大豆生长起正向调节作用。
Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.