Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China.
Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China.
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中国1,3-丁二烯接触工人DNA修复基因多态性与染色体损伤的关联性对照研究
DOI：
10.1155/2015/234675
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发表时间：
2015
影响因子：
--
通讯作者：
Ao L
中科院分区：
文献类型：
--
作者：
Xiang M;Sun L;Dong X;Yang H;Liu WB;Zhou N;Han X;Zhou Z;Cui Z;Liu JY;Cao J;Ao L
本研究旨在探讨DNA修复基因多态性与1，3-丁二烯（BD-）接触工人染色体损伤的关系。本研究在中国BD产品车间的45对职业暴露工人和行政办公室和循环水车间的配对对照工人中进行。采用在微核阻断微核（CBMN）细胞组试验中新开发的生物标志物（微核，MNi;核质桥，NPB;核芽，NBUD）检测染色体损伤。采用PCR和PCR-限制性片段长度多态性（PCR-RFLP）分析了X射线修复交叉互补组1（XRCC 1）、O 6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）、聚腺苷二磷酸-核糖聚合酶（ADPRT）和脱嘌呤/脱嘧啶核酸内切酶（APE 1）等DNA修复基因的多态性。与对照组相比，BD暴露工人表现出MNi和NPB的频率增加。结果还表明，携带XRCC 1双倍型TCGA-CCGG的BD作业工人，（4.25 ± 2.06‰）（FR = 2.10，95% CI：1.03-4.28）和TCGG-TCGA（5.80 ± 3.56‰）（FR = 2.75，95% CI：0.76-2.65）的NBUD频率显著高于TCGG-TCGG（1.89 ± 1.27‰）。本研究提示XRCC 1基因多态性可能影响BD作业工人的染色体损伤。
The aim of the study was to examine the association between polymorphisms of DNA repair genes and chromosomal damage of 1,3-butadiene- (BD-) exposed workers. The study was conducted in 45 pairs of occupationally exposed workers in a BD product workshop and matched control workers in an administrative office and a circulatory water workshop in China. Newly developed biomarkers (micronuclei, MNi; nucleoplasmic bridges, NPBs; nuclear buds, NBUDs) in the cytokinesis-blocked micronucleus (CBMN) cytome assay were adopted to detect chromosomal damage. PCR and PCR-restriction fragment length polymorphism (RFLP) are adopted to analyze polymorphisms of DNA repair genes, such as X-ray repair cross-complementing Group 1 (XRCC1), O6-methylguanine-DNA methyltransferase (MGMT), poly (adenosine diphosphate-ribose) polymerases (ADPRT), and apurinic/apyrimidinic endonucleases (APE1). The BD-exposed workers exhibited increased frequencies of MNi and NPBs when compared to subjects in the control group. The results also show that the BD-exposed workers carrying XRCC1 diplotypes TCGA-CCGG (4.25 ± 2.06‰) (FR = 2.10, 95% CI: 1.03–4.28) and TCGG-TCGA (5.80 ± 3.56‰) (FR = 2.75, 95% CI: 0.76–2.65) had statistically higher NBUD frequencies than those who carried diplotype TCGG-TCGG (1.89 ± 1.27‰). Our study suggests that polymorphisms of XRCC1 gene may influence chromosomal damage in BD-exposed workers.