Changes in secreted uterine proteins associated with embryo implantation in the mouse.
Changes in secreted uterine proteins associated with embryo implantation in the mouse.
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DOI：
10.1530/jrf.0.0840539
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发表时间：
1988-11
期刊：
影响因子：
--
通讯作者：
H. Weitlauf;M. Suda-Hartman
中科院分区：
文献类型：
--
作者：
H. Weitlauf;M. Suda-Hartman
结合二维聚丙烯酰胺凝胶电泳的双标记比率法，以测量的相对变化率的生产个别分泌蛋白的小鼠子宫在开始的过程中的蜕膜化。发现蛋白质的合成和分泌速率的差异变化的特征模式与胚胎着床部位的阳性庞他明蓝反应的发展相关。将这些变化与实验诱导的蜕膜瘤的发展相关的变化进行比较，尽管模式相似，推测反映了子宫内膜转化的共同过程，但在真实的着床部位，小分子（Mr 14，000 - 20，000）酸性蛋白的子集优先增强。有人建议，这种胚胎依赖性修改与蜕膜化相关的组成性变化反映了一种形式的胚胎-母体信号反应机制，可能是重要的小鼠植入过程。
A dual-label ratio method was used in conjunction with two-dimensional polyacrylamide gel electrophoresis to measure the relative changes in rates of production of individual secreted proteins by mouse uteri at the start of the process of decidualization. A characteristic pattern of differential changes in the rate of synthesis and secretion of the proteins was found to be associated with development of a positive Pontamine Blue reaction at the site of embryo implantation. These changes were compared with those associated with development of experimentally induced deciduomata and although the patterns were similar, presumably reflecting common processes in transformation of the endometrium, there was preferential enhancement of a subset of small (Mr 14,000-20,000) acidic proteins in the authentic implantation sites. It is suggested that this embryo-dependent modification of constitutive changes associated with decidualization reflects a form of embryo-maternal signal-response mechanism that may be important for the process of implantation in mice.