1-[4-(TRIMETHYLAMINO)PHENYL]-6-PHENYLHEXA-1,3,5-TRIENE - SYNTHESIS, FLUORESCENCE PROPERTIES, AND USE AS A FLUORESCENCE PROBE OF LIPID BILAYERS
1-[4-(TRIMETHYLAMINO)PHENYL]-6-PHENYLHEXA-1,3,5-TRIENE - SYNTHESIS, FLUORESCENCE PROPERTIES, AND USE AS A FLUORESCENCE PROBE OF LIPID BILAYERS
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DOI：
10.1021/bi00529a002
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
CALLAHAN, PJ
中科院分区：
文献类型：
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作者：
PRENDERGAST, FG;HAUGLAND, RP;CALLAHAN, PJ
1-[4-（三甲氨基）苯基]-6-苯基六烯-1,3,5-三烯（TMA-DPH）是二苯基六烯（DPH）的阳离子类似物，具有与DPH大致相似的光物理性质。在溶液中，TMA-DPH的荧光寿命较短（< 1.5 ns），而TMA-DPH的荧光寿命较短（< 1.5 ns）。增加到。约。在低于脂质热转变温度（Tc）的温度下，探针嵌入脂质双分子层时，探针的温度为7ns。阳离子电荷确保探针锚定在脂-水界面上，最有可能的是DPH片段插在脂肪酰基链的上部之间。TMA-DPH和嵌入脂质双层的DPH稳态各向异性（rss）和受限各向异性（r.infin）的变化曲线相似，但r.infin。的TMA-DPH值。Tc一般为>.14，例如，在35度。1,2-二myristoylglycero-3-phosphocholine （DMPC）中的C（比较DMPC在35度时的DPH值为0.03）。C)。阳离子探针与磷脂头基团的静电相互作用似乎不会显著影响探针的表观动力学。TMA-DPH在磷脂单层动力学的研究中应该证明是有用的，例如，在天然或重组脂蛋白中。
1-[4-(Trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene (TMA-DPH), a cationic analog of diphenylhexatriene (DPH), has photophysical properties that are generally similar to those of DPH. In solution the fluorescence lifetime (.tau.) of TMA-DPH is short (< 1.5 ns) but .tau. increases to .apprx. 7 ns when the probe is embedded in lipid bilayers at temperatures less than the thermal transition temperature (Tc) of the lipid. The cationic charge ensures that the probe is anchored at the lipid-water interface, most likely with the DPH moiety intercalated between the upper portions of the fatty acyl chains. The profiles of changes in steady-state anisotropies (rss) and limiting hindered anisotropies (r.infin.) are similar for both TMA-DPH and DPH embedded in lipid bilayers but r.infin. values for TMA-DPH even at T .mchgt. Tc are generally > 0.14, e.g., at 35.degree. C in 1,2-dimyristoylglycero-3-phosphocholine (DMPC) (cf. 0.03 for DPH in DMPC at 35.degree. C). Electrostatic interactions of the cationic probe with head groups of phospholipids do not appear to significantly influence the apparent dynamics of the probe. TMA-DPH should prove useful in the study of the dynamics of phospholipid monolayers, e.g., in native or reconstituted lipoproteins.