Human papillomavirus type 45 propagation, infection, and neutralization
Human papillomavirus type 45 propagation, infection, and neutralization
复制标题
DOI：
10.1016/s0042-6822(03)00312-x
复制
发表时间：
2003-07-20
期刊：
影响因子：
3.7
通讯作者：
Meyers, C
中科院分区：
文献类型：
--
作者：
McLaughlin-Drubin, ME;Wilson, S;Meyers, C
器官型（筏）培养系统允许研究人类乳头瘤病毒（hpv）的整个分化依赖生命周期，包括病毒粒子形态发生。我们将线性化的HPV基因组DNA引入原代角质形成细胞，在那里它循环并维持在10-50个HPV基因组DNA拷贝的范围内。在器官型培养中，随着上皮的分层和分化，病毒粒子发生形态发生。从筏培养物中纯化出HPV45病毒粒子，并能在体外感染角质形成细胞。通过检测一组HPV VLP抗血清，我们能够证明感染不仅可以与人HPV45 VLP特异性抗血清中和，也可以与人HPV18 VLP特异性抗血清中和，显示HPV18和HPV45之间的血清学交叉反应。(C) 2003 Elsevier Science（美国）版权所有。
The organotypic (raft) culture system has allowed the study of the entire differentiation-dependent life cycle of human papillomaviruses (HPVs), including virion morphogenesis. We introduced linearized HPV45 genomic DNA into primary keratinocytes, where it recircularized and maintained episomally at a range of 10-50 copies of HPV genomic DNA. Following epithelial stratification and differentiation in organotypic culture, virion morphogenesis occurred. HPV45 virions were purified from raft cultures and were able to infect keratinocytes in vitro. By testing a panel of HPV VLP antisera, we were able to demonstrate that the infection was neutralized not only with human HPV45 VLP-specific antiserum, but also with human HPV18 VLP-specific antiserum, demonstrating serological cross-reactivity between HPV18 and HPV45. (C) 2003 Elsevier Science (USA). All rights reserved.