Exploring the evolution of the proteins of the plant nuclear envelope
Exploring the evolution of the proteins of the plant nuclear envelope
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DOI：
10.1080/19491034.2016.1236166
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发表时间：
2017-01-01
期刊：
影响因子：
3.7
通讯作者：
Evans, David
中科院分区：
文献类型：
--
作者：
Poulet, Axel;Probst, Aline V.;Evans, David
在这项研究中，我们探索了高等植物核膜蛋白从最原始的植物物种到高级被子植物进化过程中的可塑性。高等植物NE含有一个核骨架和细胞骨架(LINC)复合体的功能连接物，该复合体由保守的SAD1-UNC84(SUN)结构域蛋白和植物特有的Klarsicht/ANC1/Syne同源(KASH)结构域组成。最近的证据表明，植物内膜下存在一个叶片和各种卷曲蛋白与之相关，包括密集核(CRWN；也称为LINC和NMCP)、核膜相关蛋白(NEAP)家族以及CRWN结合蛋白KAKU4。太阳结构域蛋白贯穿始终，提示太阳中期蛋白起着关键作用。Kash结构域蛋白的进化导致了复杂性的增加，一些出现在所有物种中，而另一些Kash蛋白在进化过程中逐渐获得。由于未能在研究中的单细胞生物中确定crwn同源物，以及它们在植物中的存在，我们推测，在叶片的形成过程中可能发生了收敛进化，每个王国都有新的蛋白质，如Lamin B受体(LBR)和Lamin-Emerin-Man1(LEM)结构域蛋白(动物)或neaps和KAKU4(植物)。我们的数据支持一个模型，在该模型中，核膜的复杂性通过植物谱系发生，并表明太阳中期蛋白作为核膜的早期和必要组成部分具有关键作用。
In this study, we explore the plasticity during evolution of proteins of the higher plant nuclear envelope (NE) from the most ancestral plant species to advanced angiosperms. The higher plant NE contains a functional Linker of Nucleoskeleton and Cytoskeleton (LINC) complex based on conserved Sad1-Unc84 (SUN) domain proteins and plant specific Klarsicht/Anc1/Syne homology (KASH) domain proteins. Recent evidence suggests the presence of a plant lamina underneath the inner membrane and various coiled-coil proteins have been hypothesized to be associated with it including Crowded Nuclei (CRWN; also termed LINC and NMCP), Nuclear Envelope Associated Protein (NEAP) protein families as well as the CRWN binding protein KAKU4. SUN domain proteins appear throughout with a key role for mid-SUN proteins suggested. Evolution of KASH domain proteins has resulted in increasing complexity, with some appearing in all species considered, while other KASH proteins are progressively gained during evolution. Failure to identify CRWN homologs in unicellular organisms included in the study and their presence in plants leads us to speculate that convergent evolution may have occurred in the formation of the lamina with each kingdom having new proteins such as the Lamin B receptor (LBR) and Lamin-Emerin-Man1 (LEM) domain proteins (animals) or NEAPs and KAKU4 (plants). Our data support a model in which increasing complexity at the nuclear envelope occurred through the plant lineage and suggest a key role for mid-SUN proteins as an early and essential component of the nuclear envelope.