Gene expression profiles in HPV-infected head and neck cancer
Gene expression profiles in HPV-infected head and neck cancer
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DOI：
10.1002/path.2227
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发表时间：
2007-11-01
影响因子：
7.3
通讯作者：
Belbin, T. J.
中科院分区：
文献类型：
--
作者：
Schlecht, N. F.;Burk, R. D.;Belbin, T. J.
流行病学和实验室证据表明，除了烟草和酒精之外，人乳头瘤病毒（HPV）在头颈部鳞状细胞癌（HNSCC）的一个子集中发挥着重要的病因作用。为了评估HPV感染的HNSCC的分子发病机制，我们使用cDNA微阵列比较HPV阳性和阴性HNSCC肿瘤之间的基因表达模式。从纽约市中心的42名经组织学证实的HNSCC患者中收集肿瘤组织。从冷冻的肿瘤样品中提取并纯化总DNA和RNA，并使用27 323 cDNA微阵列芯片将基因表达水平与通用人参考RNA标准品进行比较。采用MY 09/11-PCR系统和RT-PCR进行HPV检测和基因分型。在29%的HNSCC肿瘤中检测到HPV。大多数仅携带HPV 16，并表达HPV 16-E6癌基因。HPV感染率在咽部肿瘤中最高（45%）。通过监督分类分析比较了区分HPV阳性和阴性肿瘤的基因表达模式，发现多基因特征预测HPV 16在原发性HNSCC中的患病率，但错误发现率较高
Epidemiological and laboratory evidence indicate that, in addition to tobacco and alcohol, human papillomaviruses (HPV) play an important aetiological role in a subset of head and neck squamous cell carcinoma (HNSCC). To evaluate the molecular pathogenesis of HPV-infected HNSCC, we compared gene expression patterns between HPV-positive and -negative HNSCC tumours using cDNA microarrays. Tumour tissue was collected from 42 histologically confirmed HNSCC patients from an inner-city area of New York. Total DNA and RNA were extracted and purified from frozen tumour samples and gene expression levels were compared to a universal human reference RNA standard using a 27 323 cDNA microarray chip. HPV detection and genotyping were performed using an MY09/11-PCR system and RT-PCR. HPV was detected in 29% of HNSCC tumours. Most harboured only HPV16 and expressed the HPV16-E6 oncogene. HPV prevalence was highest in pharyngeal tumours (45%). Gene expression patterns that differentiated HPV-positive from negative tumours were compared by supervised classification analysis, and a multiple-gene signature was found to predict HPV16 prevalence in primary HNSCC with a false discovery rate