Secondary metabolites influence Arabidopsis/Botrytis interactions:: variation in host production and pathogen sensitivity
Secondary metabolites influence Arabidopsis/Botrytis interactions:: variation in host production and pathogen sensitivity
复制标题
DOI：
10.1111/j.1365-313x.2005.02508.x
复制
发表时间：
2005-10-01
期刊：
影响因子：
7.2
通讯作者：
Denby, KJ
中科院分区：
文献类型：
--
作者：
Kliebenstein, DJ;Rowe, HC;Denby, KJ
大量研究表明，植物/病原体相互作用部分是通过植物次生代谢产物的产生和相应的病原体耐受性介导的。然而，关于特定化合物提供对病原体的抗性的能力的报道不一致。这些研究大多集中在特定病原体的单个分离株上，表明病原体对植物产生的毒素的敏感性不同。我们测试了病原体分离株之间的毒力变异性，以及植物生产和病原体耐受次生代谢产物对毒力的影响。灰葡萄孢菌株表现出不同的敏感性纯化camalexin，camalexin敏感的菌株产生较大的病变camalexin缺陷的拟南芥基因型比野生型。与此相反，camalexin不敏感的分离产生类似的野生型和camalexin缺陷的拟南芥大小的病变。拟南芥次生代谢物生物合成突变体的其他分析表明，葡萄孢属也有不同的敏感性苯丙素和芥子油苷。此外，葡萄孢属感染产生了从发展中的病变发出的次级代谢产物响应的梯度，其中所使用的葡萄孢属分离物确定积累模式。总的来说，我们的研究结果表明，拟南芥/葡萄孢属的相互作用的影响，在代谢水平上的变化，毒素生产的主机和敏感性的病原体。
Numerous studies have suggested that plant/pathogen interactions are partially mediated via plant secondary metabolite production and corresponding pathogen tolerance. However, there are inconsistent reports on the ability of particular compounds to provide resistance to a pathogen. Most of these studies have focused on individual isolates of a given pathogen, suggesting that pathogens vary in their sensitivity to plant-produced toxins. We tested variability in virulence among pathogen isolates, and the impact on this by plant production of, and pathogen tolerance to, secondary metabolites. Botrytis cinerea isolates showed differing sensitivity to purified camalexin, and camalexin-sensitive isolates produced larger lesions on camalexin-deficient Arabidopsis genotypes than on the wild type. In contrast, the camalexin-insensitive isolate produced lesions of similar size on wild-type and camalexin-deficient Arabidopsis. Additional analysis with Arabidopsis secondary metabolite biosynthetic mutants suggests that Botrytis also has variable sensitivity to phenylpropanoids and glucosinolates. Furthermore, Botrytis infection generates a gradient of secondary metabolite responses emanating from the developing lesion, with the Botrytis isolate used determining the accumulation pattern. Collectively, our results indicate that Arabidopsis/Botrytis interactions are influenced at the metabolic level by variations in toxin production in the host and sensitivity in the pathogen.