Are laboratory bioassays for allelopathy suitable for prediction of field responses?
Are laboratory bioassays for allelopathy suitable for prediction of field responses?
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DOI：
10.1023/a:1005516431969
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发表时间：
2000-09-01
影响因子：
2.3
通讯作者：
Weston, LA
中科院分区：
文献类型：
--
作者：
Inderjit;Weston, LA
化感作用研究人员经常表达的一个担忧是，许多实验室生物测定不能充分预测在田间情况下观察到的反应。由此产生的问题是：(1)设计与生态相关的生物测定应采用什么标准？(2)相互作用涉及哪些物种(作物或杂草)？(3)我们是否正在调查残留物/残留物的化感作用或与活植物的相互作用？(4)哪些植物指示物种实际上与被调查物种共生？以及(5)什么是合适的实验对照？很难设计出一种可以用来检查所有物种反应的生物检测方法。事实上，在仔细考虑生长习性、生物特征和生态生理因素之后，每个生物测定都必须专门设计来评估物种之间的相互作用。这篇论文的目的是讨论生物测定的意义，旨在研究化感作用的一个特定方面。我们的结论是，通过实验室生物测定，我们不能证明化感作用在自然环境中是可行的。研究人员应将化感作用视为多方面生态学方法中的一个组成部分，并解决关键问题以确定特定分析的相关性。由于现场相互作用和响应的复杂性，人们只能希望预测和描述在现场环境中观察到的一些因果关系。对这些主要影响的准确评估将被证明对于指导未来研究重点将是非常宝贵的。
One concern often voiced by researchers of allelopathic interactions is that many laboratory bioassays do not adequately predict the responses observed in field situations. The questions that arise are: (1) What criteria should be implemented to design ecologically relevant bioassays? (2) What species (crops or weeds) are involved in the interaction? (3) Are we investigating allelopathy of debris/residues or interactions involving living plants? (4) Which plant indicator species are actually cohabiting with the species under investigation? and (5) What are appropriate experimental controls? It is difficult to design a bioassay that can be used to examine responses in all species. In fact, each bioassay must be designed specifically to assess species interactions after careful consideration of growth habit, biotic characteristics, and ecophysiological factors. The objective of this paper is to discuss the significance of bioassays designed to study a particular aspect of allelopathy. We conclude that through a laboratory bioassay we can not demonstrate that allelopathy is operational in natural settings. An investigator should consider allelopathy as one component in a multifaceted approach to ecology and address key questions to determine the relevance of a particular assay. Due to the complexity of field interactions and responses, one can only hope to predict and describe some of the cause-and-effect relationships observed in a field setting. An accurate assessment of these main effects will prove invaluable in directing the focus of future research emphasis.