Flooding tolerance in interspecific introgression lines containing chromosome segments from teosinte (Zea nicaraguensis) in maize (Zea mays subsp mays)
Flooding tolerance in interspecific introgression lines containing chromosome segments from teosinte (Zea nicaraguensis) in maize (Zea mays subsp mays)
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DOI：
10.1093/aob/mct160
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发表时间：
2013-10-01
期刊：
影响因子：
4.2
通讯作者：
Omori, F.
中科院分区：
文献类型：
--
作者：
Mano, Y.;Omori, F.
尼加拉瓜玉米（Zea nicaraguensis）是一种常见于洪涝地区的玉米，为选育耐涝玉米提供了有用的种质资源。玉米亚种mays）。本研究的目的是利用导入系（IL）文库筛选耐盐品系，每个导入系含有来自Z的染色体片段。为了产生IL，从玉米自交系Mi 29和Z. nicaraguensis与Mi29回交三次，自花授粉四次，并使用简单序列重复标记进行基因分型。通过回交和自交，获得了45个几乎覆盖整个玉米基因组的离子导入系。通过对叶片损伤的评价，从IL中鉴定出5个耐干旱品系。其中，IL 18系含有一个Z.位于第4号染色体长臂上的尼加拉瓜染色体片段对水淹的耐受性最大，表明该区域存在主要数量性状位点（QTL）。QTL的存在被验证，通过检查洪水的耐受性在一个群体中分离的候选区域的染色体4。结果表明，在还原性土壤条件下，诱导系形成通气组织的能力与耐淹性之间没有显著的相关性，表明诱导系中还存在其他与耐淹性相关的因素。此外，由于Z. nicaraguensis几乎覆盖了整个基因组，Z. nicaraguensis具有一些与耐淹性相关的独特性状，这些离子导入系将为进一步的QTL检测和耐淹玉米品系的开发提供有价值的材料。
Nicaraguan teosinte (Zea nicaraguensis), a species found in frequently flooded areas, provides useful germplasm for breeding flooding-tolerant maize (Z. mays subsp. mays). The objective of this study was to select flooding-tolerant lines using a library of introgression lines (ILs), each containing a chromosome segment from Z. nicaraguensis in the maize inbred line Mi29.To produce the ILs, a single F-1 plant derived from a cross between maize Mi29 and Z. nicaraguensis was backcrossed to Mi29 three times, self-pollinated four times and genotyped using simple sequence repeat markers. Flooding tolerance was evaluated at the seedling stage under reducing soil conditions.By backcrossing and selfing, a series of 45 ILs were developed covering nearly the entire maize genome. Five flooding-tolerant lines were identified from among the ILs by evaluating leaf injury. Among these, line IL18, containing a Z. nicaraguensis chromosome segment on the long arm of chromosome 4, showed the greatest tolerance to flooding, suggesting the presence of a major quantitative trait locus (QTL) in that region. The presence of the QTL was verified by examining flooding tolerance in a population segregating for the candidate region of chromosome 4. There was no significant relationship between the capacity to form constitutive aerenchyma and flooding tolerance in the ILs, indicating the presence of other factors related to flooding tolerance under reducing soil conditions.A flooding-tolerant genotype, IL18, was identified; this genotype should be useful for maize breeding. In addition, because the chromosome segments of Z. nicaraguensis in the ILs cover nearly the entire genome and Z. nicaraguensis possesses several unique traits related to flooding tolerance, the ILs should be valuable material for additional QTL detection and the development of flooding-tolerant maize lines.