Synergistic Effects of Selenium Nanoparticles and Vitamin E on Growth, Immune-Related Gene Expression, and Regulation of Antioxidant Status of Nile Tilapia (Oreochromis niloticus)
Synergistic Effects of Selenium Nanoparticles and Vitamin E on Growth, Immune-Related Gene Expression, and Regulation of Antioxidant Status of Nile Tilapia (Oreochromis niloticus)
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DOI：
10.1007/s12011-019-01857-6
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发表时间：
2020-06-01
影响因子：
3.9
通讯作者：
Helal, Azmy I.
中科院分区：
文献类型：
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作者：
Dawood, Mahmoud A. O.;Zommara, Mohsen;Helal, Azmy I.
本研究旨在探讨纳米硒（Nano Se）和/或维生素E（VE）对尼罗罗非鱼生长性能、血液健康、肠道组织形态学、氧化状态和免疫相关基因表达的影响。分别以0、1 mg纳米硒/kg、100 mg维生素E/kg和1 mg纳米硒/kg + 100 mg维生素E的饲料添加纳米硒或/和维生素E 8周。添加纳米硒和维生素E组的鱼体增重显著（P < 0.05），而添加纳米硒或纳米硒和维生素E组的鱼体增重率和增重率显著（P < 0.05）高于其他组。添加纳米硒和维生素E组鱼体的饲料转化率最低（P < 0.05），而添加维生素E组鱼体的饲料转化率最高（P < 0.05）。添加纳米硒或/和维生素E的鱼肠道形态计量学指标（绒毛长度和宽度）显著（P < 0.05）最高，杯状细胞数量最多。添加纳米硒和/或维生素E的鱼血液血液学和生化指标均正常，除血液总蛋白含量显著升高（P < 0.05）外，其他指标差异不显著。添加纳米硒或纳米硒与VE联用显著提高了GPX、SOD、CAT、NBT、溶菌酶和吞噬功能（P < 0.05），降低了MDA含量。纳米硒或纳米硒与维生素E联用组肝、脾TNF-α和IL-1 β表达显著上调（P < 0.05）。因此，纳米Se或/和VE可以有效地用于罗非鱼饲料中，以改善生长、肠道健康、血液健康、氧化状态和免疫相关基因表达。
The present study was conducted to investigate the effects of nano-selenium (Nano Se) or/and vitamin E (VE) on growth performance, blood health, intestinal histomorphology, oxidative status, and immune-related gene expression of Nile tilapia. Nano Se or/and VE at a rate of 0, 1 mg Nano Se/kg, 100 mg VE/kg, and 1 mg Nano Se/kg + 100 mg VE diet were fed to fish for 8 weeks. FBW was significantly (P < 0.05) increased in fish fed with Nano Se and VE, while fish fed with Nano Se or Nano Se and VE diets displayed significantly (P < 0.05) higher WG and SGR than the other groups. The lowest FCR was significantly (P < 0.05) detected in fish fed with Nano Se and VE, while the highest value was observed in fish VE diet. The intestinal morphometry (villi length and width) of fish fed with Nano Se or/and VE reported significantly (P < 0.05) the highest values with high number of goblet cells. Blood hematology and biochemistry parameters of fish fed with Nano Se or/and VE showed normal values with insignificant differences except for the blood total protein increased in fish fed with Nano Se or/and VE (P < 0.05). Dietary Nano Se or Nano Se and VE significantly (P < 0.05) increased the GPX, SOD, CAT, NBT, lysozyme, and phagocytosis values with decreased MDA. Liver and spleen TNF-alpha and IL-1 beta expressions were significantly (P < 0.05) upregulated in fish fed on Nano Se or Nano Se and VE. Thus, Nano Se or/and VE can be used effectively in tilapia diets for improving the growth, intestinal health, blood health, oxidative status, and immune-related gene expression.