Morphogenesis of liposomes encapsulating actin depends on the type of actin-crosslinking
Morphogenesis of liposomes encapsulating actin depends on the type of actin-crosslinking
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DOI：
10.1006/jmbi.1999.2592
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发表时间：
1999-03-26
影响因子：
5.6
通讯作者：
Hotani, H
中科院分区：
文献类型：
--
作者：
Honda, M;Takiguchi, K;Hotani, H
为了研究其内部的细胞骨架网络的组织所造成的细胞的形态发生，我们的特点是转化的脂质体封装肌动蛋白及其交联蛋白，肌成束蛋白，α-辅肌动蛋白，或细丝蛋白，使用实时高强度暗场显微镜。随着温度的升高，包封的G-肌动蛋白聚合成肌动蛋白丝并形成束或凝胶，这取决于共包封的肌动蛋白交联蛋白的类型，从而引起脂质体的各种形态变化。转化脂质体之间的形态差异表明肌动蛋白交联蛋白通过组织其特定的肌动蛋白网络来决定脂质体的形状。形态学分析表明，交联方式，即相邻的交联肌动蛋白丝之间的距离和角度的灵活性，是必不可少的形态，而不是他们的结合亲和力和化学计量肌动蛋白丝。(C)北京：科学出版社.
To study the morphogenesis of cells caused by the organization of their internal cytoskeletal network, we characterized the transformation of liposomes encapsulating actin and its crosslinking proteins, fascin, alpha-actinin, or filamin, using real-time high-intensity dark-field microscopy. With increasing temperature, the encapsulated G-actin polymerized into actin filaments and formed bundles or gels, depending on the type of actin-crosslinking protein that was co-encapsulated, causing various morphological changes of liposomes. The differences in morphology among transformed liposomes indicate that actin-crosslinking proteins determine liposome shape by organizing their specific actin networks. Morphological analysis reveals that the crosslinking manner, i.e. distance and angular flexibility between adjacent crosslinked actin filaments, is essential for the morphogenesis rather than their binding affinity and stoichiometry to actin filaments. (C) 1999 Academic Press.