Hollow, Rough, and Nitric Oxide-Releasing Cerium Oxide Nanoparticles for Promoting Multiple Stages of Wound Healing
Hollow, Rough, and Nitric Oxide-Releasing Cerium Oxide Nanoparticles for Promoting Multiple Stages of Wound Healing
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中空、粗糙且释放一氧化氮的氧化铈纳米颗粒可促进伤口愈合的多个阶段
DOI：
10.1002/adhm.201900256
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发表时间：
2019-07-10
影响因子：
10
通讯作者：
Zhang, Haiyuan
中科院分区：
文献类型：
--
作者：
Ma, Xiaomin;Cheng, Yan;Zhang, Haiyuan
伤口愈合是一个复杂而连续的生物过程，涉及多个阶段。尽管应用了各种纳米材料来加速伤口愈合过程，但在此过程中只促进了单一阶段，缺乏层次刺激。本文中，具有粗糙表面和内部L-精氨酸（AhCeO 2 NPs）的中空CeO 2纳米颗粒（NPs）被开发为用于顺序促进止血、炎症和增殖阶段的紧凑且可编程的纳米系统。AhCeO 2纳米颗粒的粗糙表面作为纳米桥迅速闭合伤口，促进止血阶段。AhCeO 2纳米颗粒的中空结构使光在颗粒内部发生多重反射，显著提高了光收集效率和电子空穴对丰度。同时，纳米颗粒的多孔外壳有利于电子-空穴的分离和活性氧的产生，在炎症阶段防止伤口感染，促进伤口愈合。AhCeO 2纳米粒的仿酶特性可减轻创面的氧化损伤，释放的L-精氨酸可在诱导型NO合酶的催化下转化为一氧化氮（NO），两者均促进增殖阶段。一系列体外和体外生物学评估证实了AhCeO 2纳米颗粒在伤口愈合过程中的有效性。
Wound healing is a complex and sequential biological process that involves multiple stages. Although various nanomaterials are applied to accelerate the wound healing process, only a single stage is promoted during the process, lacking hierarchical stimulation. Herein, hollow CeO2 nanoparticles (NPs) with rough surface and l-arginine inside (AhCeO2 NPs) are developed as a compact and programmable nanosystem for sequentially promoting the hemostasis, inflammation, and proliferation stages. The rough surface of AhCeO2 NPs works as a nanobridge to rapidly closure the wounds, promoting the hemostasis stage. The hollow structure of AhCeO2 NPs enables the multireflection of light inside particles, significantly enhancing the light harvest efficiency and electron-hole pair abundance. Simultaneously, the porous shell of AhCeO2 NPs facilitates the electron-hole separation and reactive oxygen species production, preventing wound infection and promotion wound healing during the inflammation stage. The enzyme mimicking property of AhCeO2 NPs can alleviate the oxidative injury in the wound, and the released l-arginine can be converted into nitric oxide (NO) under the catalysis of inducible NO synthase, both of which promote the proliferation stage. A series of in vitro and in vitro biological assessments corroborate the effectiveness of AhCeO2 NPs in the wound healing process.