Parameters of humoral and cellular immunity following vaccination of pigs with a European modified-live strain of porcine reproductive and respiratory syndrome virus (PRRSV)
Parameters of humoral and cellular immunity following vaccination of pigs with a European modified-live strain of porcine reproductive and respiratory syndrome virus (PRRSV)
复制标题
DOI：
10.1089/088282403322396136
复制
发表时间：
2003-01-01
期刊：
影响因子：
2.2
通讯作者：
Schmoll, F
中科院分区：
文献类型：
--
作者：
Sipos, W;Duvigneau, C;Schmoll, F
在实验感染欧洲猪繁殖与呼吸综合征病毒（PRRSV，DV株）的猪中研究了体液和细胞免疫的参数。采用实时荧光定量RT-PCR方法检测猪繁殖与呼吸综合征病毒（PRRSV），采用ELISA试剂盒检测猪PRRSV特异性抗体。使用RT-PCR在mRNA水平定量白细胞介素IL-1 α、IL-2、IL-4、IL-6、IL-8、IL-10、肿瘤坏死因子（TNF-α）和干扰素-γ（IFN-γ）以及IL-2受体（IL-2 R）。使用流式细胞术测定血液淋巴细胞亚群。未发现细胞因子表达或CD 4和CD 8标志物变化的显著变化，但在所有接种疫苗的动物中均观察到关于CD 8单阳性T细胞的相似曲线图，感染后第7天和第14天（PID）之间初始减少和增加。在接种组中，TNF-α和IL-6在PID 22和40时趋于增加，而IFN-γ未观察到增加。当将体内免疫应答与体外实验中观察到的免疫应答进行比较时，可以观察到CD 4/CD 8淋巴细胞亚群的类似变化。然而，细胞因子曲线图并不能反映体外结果。
Parameters of humoral and cellular immunity were investigated in pigs experimentally infected with a modified-live European porcine reproductive and respiratory syndrome virus (PRRSV, strain DV). PRRSV was detected by real-time RT-PCR and PRRSV-specific antibodies by a commercial ELISA test-kit, respectively. Interleukins IL-1alpha, IL-2, IL-4, IL-6, IL-8, IL-10, tumor necrosis factor (TNF-alpha) and interferon-gamma (IFN-gamma) as well as IL-2 receptor (IL-2R) were quantified at mRNA level using RT-PCR. Subpopulations of blood lymphocytes were assayed using flow cytometry. No significant changes neither in cytokine expression nor in shifts of CD4 and CD8 markers could be found, but similar curve diagrams concerning CD8 single positive T cells could be observed in all vaccinated animals with an initial decrease and an increase between post-infection days (PIDs) 7 and 14. In the vaccination group, TNF-alpha and IL-6 tended to be increased at PIDs 22 and 40, whereas no increase could be seen in IFN-gamma. When comparing the in vivo immune response to that being seen in in vitro experiments, similar shifts of CD4/CD8 lymphocyte subpopulations may be seen. Cytokine curve diagrams, however, do not reflect the in vitro findings to that extent.