Emergence of canine parvovirus type 2c in domestic dogs and cats from Thailand
Emergence of canine parvovirus type 2c in domestic dogs and cats from Thailand
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DOI：
10.1111/tbed.13177
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发表时间：
2019-07-01
影响因子：
4.3
通讯作者：
Amonsin, Alongkorn
中科院分区：
文献类型：
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作者：
Charoenkul, Kamonpan;Tangwangvivat, Ratanaporn;Amonsin, Alongkorn
犬细小病毒2型（CPV-2）是引起犬和野生动物出血性肠炎的重要病原。2000年初，犬细小病毒2c型（CPV-2c）首次被报道，随后成为欧洲和美洲流行的主要亚型。亚洲也报告了CPV-2c，包括中国、印度、台湾和越南的病例。然而，CPV-2c从未在泰国报告过。在这项研究中，我们在2016-2018年期间对泰国的狗和猫进行了病毒性肠道疾病监测。在20个月的监测期间，从有和无临床体征的犬（n = 444）和猫（n = 63）中采集了507份直肠拭子样本。采用细小病毒VP 2基因特异性PCR检测样品中的细小病毒。结果表明，犬细小病毒（CPV）的阳性率为29.95%，猫细小病毒（FPV）的阳性率为58.73%。在这项研究中，我们通过VP 2基因测序对34种细小病毒进行了鉴定。此外，通过全基因组测序对两种Thai-CPV-2（Dog/CU-24和Cat/CU-21）进行了表征。系统发育分析结果表明，Thai-CPV-2与亚洲CPV-2c的核苷酸同源性最高，与北美CPV-2c和欧洲CPV-2c的核苷酸同源性最高，但与亚洲CPV-2c的核苷酸同源性最高，与亚洲CPV-2c的核苷酸同源性最高，与亚洲CPV-2c的核苷酸同源性最高，与欧洲CPV-2c的核苷酸同源性最高。同样，全基因组分析表明，Thai-CPV与Asian-CPV-2c密切相关，在位置297 A，324 I，370 R和426 E处具有独特的氨基酸。总之，我们的结果证明了亚洲CPV-2c在泰国的狗和猫中的出现。因此，应进一步在更大范围内对家犬和家猫中的CPV-2进行监测，以确定该国和东南亚地区的主要变体及其分布的动态。
Canine parvovirus type 2 (CPV-2) is an important pathogen causing haemorrhagic enteritis in domestic dogs and wildlife worldwide. In early 2000, canine parvovirus type 2c (CPV-2c) was first reported and subsequently became a predominant subtype circulating in Europe and the Americas. CPV-2c has also been reported in Asia, including cases in China, India, Taiwan and Vietnam. However, CPV-2c has never been reported in Thailand. In this study, we conducted viral enteric disease surveillance in dogs and cats in Thailand during 2016-2018. During 20 months of surveillance, 507 rectal swab samples were collected from dogs (n = 444) and cats (n = 63) with and without clinical signs. The samples were examined for parvovirus by using VP2 gene-specific PCR for parvovirus. Our results showed that the positivity of canine parvovirus (CPV) was 29.95% and that of feline parvovirus (FPV) was 58.73%. In this study, we characterized 34 parvoviruses by VP2 gene sequencing. Moreover, two Thai-CPV-2 (Dog/CU-24 and Cat/CU-21) were characterized by whole genome sequencing. The phylogenetic results showed that Thai-CPV-2 had the highest nucleotide identities and clustered with Asian-CPV-2c but were in separate subclusters from the North American and European CPV-2c. Similarly, whole genome analyses showed that Thai-CPVs are closely related to Asian-CPV-2c, with unique amino acids at positions 297A, 324I, 370R and 426E. In summary, our results demonstrated the emergence of Asian-CPV-2c in dogs and cats in Thailand. Thus, the surveillance of CPV-2 in domestic dogs and cats should be further conducted on a larger scale to determine the dynamics of predominant variants and their distributions in the country and in the Southeast Asia region.