Black Cohosh Ameliorates Metabolic Disorders in Female Ovariectomized Rats.
Black Cohosh Ameliorates Metabolic Disorders in Female Ovariectomized Rats.
复制标题
DOI：
10.1089/rej.2015.1724
复制
发表时间：
2016-06
影响因子：
2.6
通讯作者：
Yu Sun;Qiuxiao Yu;Qiyang Shen;W. Bai;Jihong Kang
中科院分区：
文献类型：
--
作者：
Yu Sun;Qiuxiao Yu;Qiyang Shen;W. Bai;Jihong Kang
雌激素缺乏与更年期女性的代谢紊乱有关。黑升麻已被广泛用作治疗更年期综合症的替代疗法。然而，它在新陈代谢中的作用需要定义。本研究的目的是研究黑升麻对绝经后大鼠模型葡萄糖和脂质代谢的长期影响。对成年雌性 Sprague-Dawley 大鼠进行假手术 (SHAM)、卵巢切除 (OVX)、用戊酸雌二醇治疗 OVX (OVX + E) 或用异丙醇黑升麻提取物治疗 OVX (OVX + iCR)。监测动物的体重、身体成分和血糖水平。治疗3个月后处死大鼠。实验结束时，OVX+iCR和OVX+E大鼠与OVX大鼠相比，体重增加、身体和腹部脂肪量、血清甘油三酯水平、肝脏脂肪堆积和脂肪细胞肥大均显着降低。此外，OVX + iCR 大鼠的胰岛素抵抗和葡萄糖耐受不良得到改善，但 OVX + E 大鼠则没有改善。在 OVX + iCR 动物中未检测到肝毒性。此外，蛋白质印迹分析表明 OVX + iCR 和 OVX + E 大鼠脂肪组织中的脂肪分解增加。使用培养的原代大鼠脂肪细胞进行的体外实验数据也表明，黑升麻可以影响脂肪细胞的脂肪分解。总之，长期服用适当剂量的黑升麻可改善 OVX 大鼠的代谢紊乱。因此，该药物有望用于治疗绝经期和绝经后妇女的代谢紊乱。
Estrogen deficiency is associated with metabolic derangements in menopausal women. Black cohosh has been widely used as an alternative therapy in the treatment of menopausal syndrome. However, its role in metabolism needs to be defined. The aim of the present study was to investigate the long-term effect of black cohosh on glucose and lipid metabolism in a rat model of post-menopause. Adult female Sprague-Dawley rats were sham operated (SHAM), ovariectomized (OVX), OVX with the treatment of estradiol valerate (OVX + E), or OVX with the treatment of isopropanolic black cohosh extract (OVX + iCR). Body weight, body composition, and blood glucose levels of the animals were monitored. The rats were then sacrificed after 3 months of the treatments. At the end of the experiment, OVX + iCR and OVX + E rats exhibited a significant decrease in body weight gain, body and abdominal fat mass, serum triglycerides levels, hepatic fat accumulation, and adipocyte hypertrophy compared with OVX rats. In addition, insulin resistance and glucose intolerance were improved in OVX + iCR but not in OVX + E rats. No hepatotoxicity was detected in OVX + iCR animals. Furthermore, western blot analysis suggested the increased lipolysis in adipose tissue of OVX + iCR and OVX + E rats. Data from in vitro experiments using cultured primary rat adipocytes also showed that black cohosh could affect lipolysis of adipocytes. In conclusion, the long-term treatment of black cohosh at a proper dosage ameliorated metabolic derangements in OVX rats. Thus, this drug is promising for the treatment of metabolic disorders in menopausal and post-menopausal women.