Normalization of Host Intestinal Mucus Layers Requires Long-Term Microbial Colonization.
Normalization of Host Intestinal Mucus Layers Requires Long-Term Microbial Colonization.
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DOI：
10.1016/j.chom.2015.10.007
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发表时间：
2015-11-11
影响因子：
30.3
通讯作者：
Hansson GC
中科院分区：
文献类型：
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作者：
Johansson ME;Jakobsson HE;Holmén-Larsson J;Schütte A;Ermund A;Rodríguez-Piñeiro AM;Arike L;Wising C;Svensson F;Bäckhed F;Hansson GC
肠道黏液层提供了一个屏障，限制细菌与下层上皮的接触。黏液结构由肠道位置和微生物群决定。为了了解共生菌是如何调节肠道粘液的，我们研究了无菌条件下和微生物定植过程中的粘液特性。虽然GF小鼠的结肠粘液结构与常规饲养（Convr）小鼠相似，但GF内黏液层可以穿透细菌大小的珠子。在定植过程中，用Convr菌群灌胃GF小鼠，小肠粘液需要5周才能正常分离，结肠内粘液需要6周才能变得不可穿透。在定植期间，小肠微生物群的组成与Convr供体相似，直到三周时，拟杆菌增加，厚壁菌门减少，节段丝状细菌无法检测到。这些发现强调了黏液层发育的动力学，并表明成熟微生物-黏液相互作用的研究应该在定植后几周进行。
The intestinal mucus layer provides a barrier limiting bacterial contact with the underlying epithelium. Mucus structure is shaped by intestinal location and the microbiota. To understand how commensals modulate gut mucus, we examined mucus properties under germ-free (GF) conditions and during microbial colonization. Although the colon mucus structure of GF mice was similar to conventionally raised (Convr) mice, the GF inner mucus layer was penetrable to bacteria-sized beads. During colonization, in which GF mice were gavaged with Convr microbiota, the small intestine mucus required five weeks to be normally detached and colonic inner mucus six weeks to become impenetrable. The composition of the small intestinal microbiota during colonization was similar to Convr donors until three weeks when Bacteroides increased, Firmicutes decreased, and segmented filamentous bacteria became undetectable. These findings highlight the dynamics of mucus layer development and indicate that studies of mature microbe-mucus interactions should be conducted weeks after colonization.