3D Bioprinting using UNIversal Orthogonal Network (UNION) Bioinks
3D Bioprinting using UNIversal Orthogonal Network (UNION) Bioinks
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DOI：
10.1002/adfm.202007983
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发表时间：
2020-11-20
影响因子：
19
通讯作者：
Heilshorn, Sarah C.
中科院分区：
文献类型：
--
作者：
Hull, Sarah M.;Lindsay, Christopher D.;Heilshorn, Sarah C.
三维(3D)生物打印是一种很有前途的组织状结构打印技术，但生物墨水缺乏多样性是一个主要限制。理想情况下，每种细胞类型都可以用自己定制的生物墨水打印。为了满足这种普遍适用的生物墨水策略的需要，开发了一种通用的生物正交生物墨水交联机制，该机制与细胞兼容并与一系列聚合物一起工作。这一系列材料被称为通用、正交网(Union)生物油墨。为了展示Union生物墨水的多功能性，明胶、透明质酸(HA)、重组弹性蛋白样蛋白(ELP)和聚乙二醇(PEG)分别用作主干聚合物，以创建存储模数从200到10000pa的墨水。因为联合生物墨水是通过一种共同的化学物质进行交联的，所以多种材料可以打印在一起，形成统一的粘合结构。这种方法与任何支持联盟交联剂扩散的支撑浴都是兼容的。黏附基质的人角膜间充质基质细胞和非黏附基质的人诱导多能干细胞来源的神经前体球体都印有Union生物墨水。细胞在打印后仍保持较高的存活率，并表达特有的表型标志物。因此，Union生物墨水是一种多用途的策略，可以扩展可用于3D生物打印的定制材料工具包。
Three-dimensional (3D) bioprinting is a promising technology to produce tissue-like structures, but a lack of diversity in bioinks is a major limitation. Ideally each cell type would be printed in its own customizable bioink. To fulfill this need for a universally applicable bioink strategy, a versatile bioorthogonal bioink crosslinking mechanism that is cell compatible and works with a range of polymers is developed. This family of materials is termed UNIversal, Orthogonal Network (UNION) bioinks. As demonstration of UNION bioink versatility, gelatin, hyaluronic acid (HA), recombinant elastin-like protein (ELP), and polyethylene glycol (PEG) are each used as backbone polymers to create inks with storage moduli spanning from 200 to 10 000 Pa. Because UNION bioinks are crosslinked by a common chemistry, multiple materials can be printed together to form a unified, cohesive structure. This approach is compatible with any support bath that enables diffusion of UNION crosslinkers. Both matrix-adherent human corneal mesenchymal stromal cells and non-matrix-adherent human induced pluripotent stem cell-derived neural progenitor spheroids are printed with UNION bioinks. The cells retained high viability and expressed characteristic phenotypic markers after printing. Thus, UNION bioinks are a versatile strategy to expand the toolkit of customizable materials available for 3D bioprinting.