Improving bacteria viability in metal oxide hostsvia an alginate-based hybrid approach
Improving bacteria viability in metal oxide hostsvia an alginate-based hybrid approach
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DOI：
10.1039/c1jm10684h
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发表时间：
2011-05
影响因子：
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通讯作者：
M. Perullini;Makhlouf Amoura;M. Jobbágy;C. Roux;J. Livage;T. Coradin;S. A. Bilmes
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文献类型：
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作者：
M. Perullini;Makhlouf Amoura;M. Jobbágy;C. Roux;J. Livage;T. Coradin;S. A. Bilmes
在薄水铝石和氧化锆颗粒存在的情况下，将细胞包封在海藻酸盐珠中，然后由胶体金属氧化物进行无机凝胶化的两步工艺成功应用于大肠杆菌的固定化。在al基凝胶的情况下，在低生物聚合物含量下获得的藻酸盐珠提供了一个有效的屏障来抵抗包封应力。相比之下，藻酸盐浓度的增加以及磷酸盐诱导的生物聚合物珠的矿化被发现是维持被困细菌在锆基凝胶中的生存能力所必需的。利用模型分子和胶体进行的扩散研究表明，带正电荷的zr -低聚物和ZrO2纳米颗粒可能参与了前体溶液的细胞毒性。这种封装过程的优化允许首次观察e.c oli在这种金属氧化物-海藻酸盐混合凝胶中生长。在这项工作中提出的结果给出了一个明确的证据，溶胶-凝胶为基础的细胞封装现在可以设想在各种金属氧化物宿主通过预封装环境的优化。
A two-step process relying on cell encapsulation in alginate beads followed by inorganic gelation from colloidal metal oxides was successfully applied to the immobilization of Escherichia coli bacteria in the presence of boehmite and zirconium oxyhydroxide particles. In the case of the Al-based gel, the alginate bead obtained at low biopolymer content provides an efficient barrier against the encapsulation stress. In contrast, an increase in the alginate concentration together with the phosphate-induced mineralization of the biopolymer bead is found necessary to maintain the viability of entrapped bacteria in Zr-based gels. Diffusion studies using model molecular and colloidal species put in evidence that positively charged Zr-oligomers and ZrO2 nanoparticles may be involved in the cytotoxicity of the precursor solution. This optimization of the encapsulation process allows the first observation of E. coligrowth within such metal oxide–alginate hybrid gels. Results presented in this work give a clear evidence that sol–gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.