Borrelia burgdorferi genes selectively expressed in the infected host.
Borrelia burgdorferi genes selectively expressed in the infected host.
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伯氏疏螺旋体基因在受感染的宿主中选择性表达。
DOI：
10.1073/pnas.92.10.4269
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发表时间：
1995
影响因子：
11.1
通讯作者：
Fikrig,E
中科院分区：
文献类型：
--
作者：
Suk,K;Das,S;Sun,W;Jwang,B;Barthold,SW;Flavell,RA;Fikrig,E
免疫学筛选策略用于选择仅在宿主中表达的微生物基因。对伯氏疏螺旋体（莱姆病病原体）表达文库的差异筛选鉴定了编码20.7-kDa抗原的基因（p21），该抗原与来自活动性感染小鼠的血清中的抗体反应，但不与来自用热灭活B免疫的小鼠的血清反应。burgdorferi。通过北方印迹分析和RNA PCR证实了p21在感染宿主中的选择性表达。表达文库的进一步差异筛选鉴定了至少5个额外的B。Burgdorferi基因在体内选择性表达。该筛选方法可用于鉴定在多种病原微生物中体内诱导的基因，对于这些病原微生物，基因转移系统目前不可用。
An immunological screening strategy was used to select microbial genes expressed only in the host. Differential screening of a Borrelia burgdorferi (the Lyme disease agent) expression library identified a gene (p21) encoding a 20.7-kDa antigen that reacted with antibodies in serum from actively infected mice but not serum from mice immunized with heat-killed B. burgdorferi. Selective expression of p21 in the infected host was confirmed by Northern blot analysis and RNA PCR. Further differential screening of the expression library identified at least five additional B. burgdorferi genes are selectively expressed in vivo. This screening method can be used to identify genes induced in vivo in a wide variety of pathogenic microorganisms for which a gene transfer system is not currently available.