Combined dermal exposure to permethrin and cis-urocanic acid suppresses the contact hypersensitivity response in C57BL/6N mice in an additive manner.
Combined dermal exposure to permethrin and cis-urocanic acid suppresses the contact hypersensitivity response in C57BL/6N mice in an additive manner.
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皮肤接触氯菊酯和顺式尿刊酸的组合可以叠加方式抑制 C57BL/6N 小鼠的接触性超敏反应。
DOI：
10.1016/j.jphotobiol.2004.09.004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Holladay,SD
中科院分区：
文献类型：
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作者：
Prater,MR;Blaylock,BL;Holladay,SD
皮肤暴露于拟除虫菊酯杀虫剂氯菊酯可显著抑制C57BL/6N小鼠对恶唑酮的接触性超敏反应。此外，顺式尿酸（cUCA）是一种内源性皮肤发色团，在暴露于紫外线辐射后异构为活性形式，可调节细胞介导的皮肤免疫反应。本研究描述了局部氯菊酯和皮内cUCA联合暴露后皮肤免疫的改变。雌性C57BL/6N小鼠分别每天给予5、50或100 μg的cUCA，连续5天。然后通过小鼠耳肿胀试验（MEST）对恶唑酮的反应来评估CH。与对照组相比，反应分别下降了52.3%、76.3%和76.3%。然后，小鼠分别在cUCA处理的第1、3或5天，每天分别暴露于5 μg的cUCA和1.5、5、15或25 μL的氯菊酯，以评估两种化学物质的联合免疫调节作用，或者每天暴露于cUCA 5天，在最后一次cUCA注射后的第3、5或7天分别暴露于氯菊酯，以证明持久的免疫抑制作用。最终治疗后2天，用恶唑酮致敏小鼠，并进行MEST。在cUCA注射第1天，局部注射五次cUCA和氯菊酯，与对照相比，MEST抑制率高达93.3%。与对照相比，25 μL氯菊酯对ch3、5、7 d的抑制作用分别为87.5%、86.6%和74.2%。综上所述，这些数据表明，同时暴露于三羧酸甲酯和氯菊酯会严重抑制细胞介导的皮肤免疫。
Cutaneous exposure to the pyrethroid insecticide permethrin significantly suppresses contact hypersensitivity (CH) response to oxazolone in C57BL/6N mice. Additionally, cis-urocanic acid (cUCA), an endogenous cutaneous chromophore isomerized to its active form following exposure to ultraviolet radiation, modulates cell-mediated cutaneous immune responses. This study describes cutaneous immune alterations following combined topical permethrin and intradermal cUCA exposure. Female C57BL/6N mice were administered 5, 50 or 100 μg cUCA daily for 5 consecutive days. CH was then evaluated by the mouse ear swelling test (MEST) response to oxazolone. Decreased responses of 52.3%, 76.3% and 76.3%, respectively, as compared to controls were observed. Then, mice were co-exposed to 5 μg cUCA daily for 5 days and 1.5, 5, 15, or 25 μL permethrin, on either day 1, 3 or 5 of the cUCA treatment to evaluate combined immunomodulatory effects of the two chemicals, or cUCA daily for 5 days followed by permethrin on day 3, 5, or 7 after the last cUCA injection to demonstrate prolonged immunosuppressive effects. Two days after final treatment, mice were sensitized with oxazolone and MEST was performed. Mice receiving five cUCA injections and permethrin topically on cUCA injection day 1 showed up to 93.3% suppression of MEST compared to vehicle control. CH was suppressed by 87.5%, 86.6% and 74.2% in mice treated with 25 μL permethrin on days 3, 5 and 7 after cUCA, respectively, compared to vehicle control. Taken together, these data indicate co-exposure to cUCA and permethrin profoundly suppresses cell-mediated cutaneous immunity.