FACILITATION BY PARTIAL-HEPATECTOMY OF TUMOR-GROWTH WITHIN THE RAT-LIVER FOLLOWING INTRAPORTAL INJECTION OF SYNGENEIC TUMOR-CELLS
FACILITATION BY PARTIAL-HEPATECTOMY OF TUMOR-GROWTH WITHIN THE RAT-LIVER FOLLOWING INTRAPORTAL INJECTION OF SYNGENEIC TUMOR-CELLS
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DOI：
10.1007/bf01769354
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发表时间：
1991-07-01
影响因子：
4
通讯作者：
TAYLOR, I
中科院分区：
文献类型：
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作者：
LOIZIDOU, MC;LAWRANCE, RJ;TAYLOR, I
采用三分之二肝部分切除术（PH），研究了机械创伤和再生对大鼠门静脉注射肿瘤生长的影响。当在肿瘤注射前不到2天进行PH时，肿瘤在切除瘢痕处生长（34/34只动物）。而在注射前4-7天进行PH时，肿瘤发生在再生叶内，而不是瘢痕处（50/51）。将相同剂量的细胞注射到肝脏完整的大鼠中，在12/30的动物中几乎没有肿瘤发生。门静脉内注射Cr-51标记的肿瘤细胞均匀分布在肝脏中，无论PH后的时间。PH后不同时间的肿瘤模式不是由于选择性捕获的注射细胞。肝提取物显示，表皮生长因子样活性是不变的PH，而肝素结合生长因子的活性在PH后2天达到峰值，在实质中的肿瘤生长的发病率增加之前。用[I-125]脱氧尿苷和溴脱氧尿苷脉冲标记，我们在PH后第1天和第4天观察到两个DNA合成峰。溴脱氧尿苷免疫组化显示第一个峰仅限于肝细胞。第二个峰涉及非肝细胞，与再生叶中增强的肿瘤摄取的开始一致。
The effects of both mechanical trauma and regeneration on the growth of intraportally injected tumor in the rat liver were investigated using two-thirds partial hepatectomy (PH). Tumor grew at the excision scar when PH was performed less than 2 days before tumor injection (34/34 animals). However, when the PH was performed 4-7 days before injection, tumor developed within the regenerating lobe, but not at the scar (50/51). Injecting the same cell dose into rats with intact livers caused few tumors to develop in 12/30 animals. Intraportally injected Cr-51-labelled tumor cells distributed uniformly in the liver irrespective of the time after PH. Patterns of tumor take seen at different times after PH were not due to selective trapping of the injected cells. Liver extracts showed that epidermal growth factor-like activity was unaltered by PH, while heparin-binding growth factor activity peaked at 2 days post-PH, before the incidence of tumor growth in the parenchyma increased. We observed two peaks of DNA synthesis at days 1 and 4 post-PH by pulse labeling with [I-125]deoxyuridine and bromodeoxyuridine. Bromodeoxyuridine immunohistochemistry showed the first peak to be confined to hepatocytes. The second peak involved non-hepatocytes and coincided with the beginning of enhanced tumor take in the regenerating lobe.