Study on molecular structural characteristics of tea polysaccharide
Study on molecular structural characteristics of tea polysaccharide
复制标题
茶多糖分子结构特征的研究
DOI：
10.1016/j.ijbiomac.2010.03.026
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发表时间：
2010-08-01
影响因子：
8.2
通讯作者：
Liang, Qin
中科院分区：
文献类型：
--
作者：
Guo, Li;Du, Xianfeng;Liang, Qin
茶多糖因其独特的生物学特性而逐渐受到人们的关注。然而，由于缺乏适当的方法，TPS的分子特性尚不清楚。为此，采用高效排粒径色谱法（HPSEC）、多角度激光散射法（MALLS）和粘度法对TPS的分子特性进行了研究。结果表明，TPS的摩尔质量为2.287 ~ 2.762 × 10(5) g mol(-1)， RMS半径为132.1 ~ 145.9 nm， M-w/M-n为1.028。建立Mark-Houwink方程为[eta] = 0.5423(w)(0.5379)，特征粘度和分子链参数为[eta] = 1.007 dLg(-1), k(H) = 0.845, k(k) = 0.387, alpha = 0.5379, k = 0.5423。此外，基于RMS半径与摩尔质量构象图的斜率为0.24 +/- 0.00，我们认为TPS的分子形态是均匀的球形聚合物，在溶液中有分支。(C) 2010 Elsevier B.V.版权所有
Tea polysaccharide (TPS) is attracting more attention gradually due to its particular biological properties. However, molecular characteristics of TPS are unclear since appropriate method is still absent. So, study on the molecular characteristics of TPS was carried out by high-performance size-exclusion chromatography (HPSEC), multi-angle laser light scattering (MALLS) and viscosimetry. The results showed that the molar masses of TPS ranged from 2.287 x 10(5) to 2.762 x 10(5) g mol(-1), the RMS radii distributed from 132.1 to 145.9 nm, and M-w/M-n is 1.028. The Mark-Houwink equation was established as [eta] = 0.5423(w)(0.5379), and the intrinsic viscosity and molecular chain parameters were as follows: [eta] = 1.007 dLg(-1), k(H) = 0.845, k(K) = 0.387, alpha = 0.5379, K= 0.5423.In addition, based on the slope of the RMS radius versus molar mass conformational plot being 0.24 +/- 0.00, we suggest that the molecular morphology of TPS is a homogeneous and spherical polymer with branch in solution. (C) 2010 Elsevier B.V. All rights reserved.