Intestinal autophagy activity is essential for host defense against Salmonella typhimurium infection in Caenorhabditis elegans
Intestinal autophagy activity is essential for host defense against Salmonella typhimurium infection in Caenorhabditis elegans
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DOI：
10.1016/j.dci.2014.03.009
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发表时间：
2014-08-01
影响因子：
2.9
通讯作者：
Jia, Kailiang
中科院分区：
文献类型：
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作者：
Curt, Alexander;Zhang, Jiuli;Jia, Kailiang
鼠伤寒沙门氏菌感染肠上皮细胞和巨噬细胞。自噬是一种存在于所有真核生物中的溶酶体降解途径。据报道，自噬可以限制秀丽隐杆线虫和哺乳动物中沙门氏菌的复制。然而，肠道自噬活性是否在宿主防御线虫沙门氏菌感染中发挥作用尚不清楚。在这项研究中，我们抑制了不同秀丽隐杆线虫组织中的自噬基因 bec-1，并检查了这些动物在沙门氏菌感染后的存活情况。在这里，我们发现抑制肠道中的 bec-1 基因而不是其他组织中的 bec-1 基因会导致对沙门氏菌感染的易感性，这与最近的小鼠研究一致，表明自噬参与肠上皮细胞中沙门氏菌的清除。因此，肠道自噬活性对于宿主防御沙门氏菌感染至关重要，从线虫到小鼠，也许在人类也是如此。 (C) 2014 Elsevier Ltd. 保留所有权利。
Salmonella typhimurium infects both intestinal epithelial cells and macrophages. Autophagy is a lysosomal degradation pathway that is present in all eukaryotes. Autophagy has been reported to limit the Salmonella replication in Caenorhabditis elegans and in mammals. However, it is unknown whether intestinal autophagy activity plays a role in host defense against Salmonella infection in C elegans. In this study, we inhibited the autophagy gene bec-1 in different C. elegans tissues and examined the survival of these animals following Salmonella infection. Here we show that inhibition of the bec-1 gene in the intestine but not in other tissues confers susceptibility to Salmonella infection, which is consistent with recent studies in mice showing that autophagy is involved in clearance of Salmonella in the intestinal epithelial cells. Therefore, the intestinal autophagy activity is essential for host defense against Salmonella infection from C elegans to mice, perhaps also in humans. (C) 2014 Elsevier Ltd. All rights reserved.