Construction and Characterization of a Doxycycline-Inducible Transgenic System in Msx2 Expressing Cells
Construction and Characterization of a Doxycycline-Inducible Transgenic System in Msx2 Expressing Cells
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DOI：
10.1002/dvg.20506
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发表时间：
2009-05-01
期刊：
影响因子：
1.5
通讯作者：
Ma, Liang
中科院分区：
文献类型：
--
作者：
Lin, Congxing;Yin, Yan;Ma, Liang
同源框基因Msx2在胚胎发生和出生后发育过程中广泛表达，并在器官发生过程中发挥重要作用。我们开发了一种 Msx2-rtTA BAC 转基因系，它可以在强力霉素 (Dox) 处理后激活 Msx2 表达细胞中的 TetO-Cre 表达。使用 Rosa26-LacZ (R26R) 报告系，我们发现 rtTA 在表达 Msx2 的器官中被激活，包括四肢、心脏、外生殖器、泌尿生殖系统、毛囊和颅面部区域。此外，我们表明，在身体附肢中，根据 Dox 治疗的时间，转基因可以在不同的区域被激活。此外，转基因还可以在毛囊和泌尿生殖系统等成人组织中有效激活。总而言之，该 Msx2-rtTA;TetO-Cre 系统是一种有价值的工具，可用于以时间和空间限制的方式研究上述器官发育中的基因功能，以及用于表达 Msx2 的细胞的组织谱系追踪。当出生后诱导时，该系统还可用于研究成人组织中的基因功能，而不会影响正常发育和模式。创世纪 47:352-359, 2009。(C) 2009 Wiley-Liss, Inc.
Homeobox gene Msx2 is widely expressed during both embryogenesis and postnatal development and plays important roles during organogenesis. We developed an Msx2-rtTA BAC transgenic line which can activate TetO-Cre expression in Msx2-expressing cells upon doxycycline (Dox) treatment. Using the Rosa26-LacZ (R26R) reporter line, we show that rtTA is activated in Msx2-expressing organs including the limb, heart, external genitalia, urogenital system, hair follicles and craniofacial regions. Moreover, we show that in body appendages, the transgene can be activated in different domains depending on the timing of Dox treatment. In addition, the transgene can also be effectively activated in adult tissues such as the hair follicle and the urogenital system. Taken together, this Msx2-rtTA;TetO-Cre system is a valuable tool for studying gene function in the development of the aforementioned organs in a temporal and spatially-restricted manner, as well as for tissue lineage tracing of Msx2-expressing cells. When induced postnatally, this system can also be used to study gene function in adult tissues without compromising normal development and patterning. genesis 47:352-359, 2009. (C) 2009 Wiley-Liss, Inc.