MACROPHAGES ARE REQUIRED FOR CELL-DEATH AND TISSUE REMODELING IN THE DEVELOPING MOUSE EYE
MACROPHAGES ARE REQUIRED FOR CELL-DEATH AND TISSUE REMODELING IN THE DEVELOPING MOUSE EYE
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DOI：
10.1016/0092-8674(93)80047-i
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发表时间：
1993-08-13
期刊：
影响因子：
64.5
通讯作者：
BISHOP, JM
中科院分区：
文献类型：
--
作者：
LANG, RA;BISHOP, JM
为了鉴定和表征巨噬细胞介导的组织重塑过程，我们培育了巨噬细胞特异性转基因白喉毒素表达的转基因小鼠。转基因的表达破坏了眼睛和腹腔中成熟巨噬细胞的亚群，并导致两种正常瞬态的眼部组织——玻璃状血管和瞳孔膜的持续存在。此外，组成瞳孔膜的细胞在结构正常重建后存活长达14天，这表明巨噬细胞主动诱导靶细胞死亡。因此，这些转基因小鼠为巨噬细胞积极参与发育程序性组织重塑提供了直接证据，并确定了眼内的玻璃状血管和瞳孔膜是巨噬细胞介导的重塑的靶点。
To identify and characterize tissue remodeling processes mediated by macrophages, we have generated transgenic mice in which diphtheria toxin is expressed from a macrophage-specific transgene. Expression of the transgene disrupts subsets of mature macrophages in both the eye and the peritoneal cavity and results in the persistence of two normally transient ocular tissues, the hyaloid vasculature and the pupillary membrane. Furthermore, the cells comprising the pupillary membrane appear alive up to 14 days after the structure is normally remodeled, suggesting that the macrophage actively elicits target cell death. Thus, these transgenic mice provide direct evidence for the active involvement of macrophages in developmentally programed tissue remodeling and identify the hyaloid vessels and the pupillary membrane in the eye as targets of macrophage-mediated remodeling.