Effect of solvent on ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry of peptides
Effect of solvent on ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry of peptides
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DOI：
10.1016/j.chemphys.2013.03.004
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发表时间：
2013-06-20
期刊：
影响因子：
2.3
通讯作者：
Awazu, Kunio
中科院分区：
文献类型：
--
作者：
Hazama, Hisanao;Furukawa, Satoshi;Awazu, Kunio
虽然已经提出了各种基质辅助激光解吸/电离（MALDI）的理论模型，但没有一个模型可以解释所有实验观察到的现象。据报道，样品制备中所用溶剂的含水量对MALDI的离子产率有影响。然而，先前提出的模型没有考虑溶剂。因此，我们研究了溶剂对多肽MALDI质谱分析的影响。在水、甲醇、乙醇、四氢呋喃和乙腈溶剂中，以水为溶剂时肽离子的信号峰面积最大。结果表明，干燥后基质晶体中残留的水溶剂对MALDI的电离过程有一定的影响。由此推断，在MALDI理论模型中加入残留溶剂的影响有助于提高模型的精度。(C) 2013 Elsevier b.v.版权所有
Although various theoretical models of matrix-assisted laser desorption/ionization (MALDI) have been proposed, no model can explain all of the experimentally observed phenomena. It has been reported that water content in the solvent used in the sample preparation has influence on the ion yield of MALDI. However, the previously proposed models do not take account of the solvent. Therefore, we have investigated the influence of the solvent on MALDI mass spectrometry of peptides. Among the solvents of water, methanol, ethanol, tetrahydrofuran, and acetonitrile, the signal peak area of the peptide ion was the largest when the water solvent was used. It is suggested that the residual water solvent contained in the dried matrix crystal has influence on the ionization processes of MALDI. It is inferred that inclusion of the influence of the residual solvent into the theoretical model of MALDI could contribute to the improvement of the precision of the model. (C) 2013 Elsevier B. V. All rights reserved.