Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida
Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida
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DOI：
10.3354/dao037089
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发表时间：
1999-07-30
影响因子：
1.4
通讯作者：
Klein, AP
中科院分区：
文献类型：
--
作者：
Lackovich, JK;Brown, DR;Klein, AP
海龟纤维乳头状瘤病（FP）是一种以良性但使人衰弱的皮肤纤维乳头状瘤和偶尔的内脏纤维瘤增殖为特征的疾病。传播实验表明，在FP的病因学中，存在于过滤的无细胞肿瘤匀浆中的氯仿敏感性转化剂。在这项研究中，共识引物PCR方法被用来测试龟疱疹病毒与纤维乳头状瘤病的关联。纤维乳头状瘤和皮肤样本，从17绿色和2个红海龟感染FP搁浅沿着佛罗里达海岸线。对19只海龟的93个皮肤和内脏肿瘤以及16只海龟的33个皮肤样本进行了测试。通过PCR检测，所有受FP影响的海龟都有与肿瘤相关的疱疹病毒。96%（89/93）的肿瘤，但只有9%（3/33）的皮肤样本，从受影响的海龟含有可检测到的疱疹病毒。含有疱疹病毒的皮肤样本都在纤维乳头状瘤的2厘米范围内。此外，1的11个瘢痕组织样本的网站，纤维乳头状瘤已被删除2至51周前从5绿色海龟含有可检测的疱疹病毒。2只绿色和2只红海龟的18份正常皮肤样品均未检出疱疹病毒。这些数据表明，疱疹病毒仅在肿瘤内或肿瘤附近是令人愉快的。为了确定是否相同的病毒感染两种龟种，疱疹病毒DNA聚合酶基因的部分核苷酸序列测定从6个红海龟和2个绿色龟样品。序列预测，疱疹病毒的红海龟不同于那些绿色海龟只有1 60个氨基酸的序列检查，表明龟疱疹病毒表现出轻微的内部变异是唯一的疱疹病毒都存在于肿瘤的绿色和红海龟。FP相关的疱疹病毒抵抗龟细胞系，支持其他龟疱疹病毒的复制培养。这些结果导致的结论是，龟疱疹病毒是定期与纤维乳头状瘤病，而不仅仅是一个偶然发现受影响的海龟。
Sea turtle fibropapillomatosis (FP) is a disease marked by proliferation of benign but debilitating cutaneous fibropapillomas and occasional Visceral fibromas. Transmission experiments have implicated a chloroform-sensitive transforming agent present in filtered cell-free tumor homogenates in the etiology of FP. In this study, consensus primer PCR methodology was used to test the association of a chelonian herpesvirus with fibropapillomatosis. Fibropapilloma and skin samples were obtained from 17 green and 2 loggerhead turtles affected with FP stranded along the Florida coastline. Ninety-three cutaneous and visceral tumors from the 19 turtles, and 33 skin samples from 16 of the turtles, were tested. All turtles affected with FP had herpesvirus associated with their tumors as detected by PCR. Ninety-six percent (89/93) of the tumors, but only 9% (3/33) of the skin samples, from affected turtles contained detectable herpesvirus. The skin samples that contained herpesvirus were all within 2 cm of a fibropapilloma. Also, 1 of 11 scar tissue samples from sites where fibropapillomas had been removed 2 to 51 wk earlier from 5 green turtles contained detectable herpesvirus. None of 18 nor mal skin samples from 2 green and 2 loggerhead turtles stranded without FP contained herpesvirus. The data indicated that herpesvirus was delectable only within or close to tumors. To determine if the same Virus infected both turtle species, partial nucleotide sequences of the herpesvirus DNA polymerase gene were determined from 6 loggerhead and 2 green turtle samples. The sequences predicted that herpesvirus of loggerhead turtles differed from those of green turtles by only 1 of 60 amino acids in the sequence examined, indicating that a chelonian herpesvirus exhibiting minor intratypic variation was the only herpesvirus present in tumors of both green and loggerhead turtles. The FP-associated herpesvirus resisted cultivation on chelonian cell lines which support the replication of other chelonian herpesviruses. These results lead to the conclusion that a chelonian herpesvirus is regularly associated with fibropapillomatosis and is not merely an incidental finding in affected turtles.