Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice
Grass‐specific ABERRANT MICROSPORE DEVELOPMENT 1 is required for maintaining pollen fertility in rice
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维持水稻花粉育性需要草特异性异常微孢子发育 1
DOI：
10.1111/tpj.15921
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Li Shuangcheng
中科院分区：
文献类型：
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作者：
Zou Ting;Xiong Pingping;Zhou Fuxing;Zhou Dan;Chen Hao;Li Gongwen;Peng Kun;Zheng Kaiyou;Han Yuhao;Zhang Kaixuan;Zhang Xu;Yang Shangyu;Deng Qiming;Wang Shiquan;Zhu Jun;Liang Yueyang;Sun Changhui;Yu Xiumei;Liu Huainian;Wang Lingxia;Li Ping;Li Shuangcheng
花粉发育包括一系列需要精确基因调控的生物学事件。虽然一些转录因子（TF）已被证明在维持花粉育性中发挥作用，但绒毡层发育和花粉壁形成的主要调控网络在很大程度上是未知的。在此，我们报告，异常小孢子萌发1（AMD1），以前作为未知的蛋白质注释，是必需的绒毡层发育和花粉外壁图案在水稻（Oryza sativa L.）。AMD1编码一种草特异性蛋白，在细胞核中表现出反式激活活性，并在花粉发育过程中在绒毡层和小孢子中时空表达。进一步的生物化学分析表明，AMD 1直接激活缺陷花粉壁（DPW）和聚酮合成酶2（OsPKS2）的转录，这两种酶都参与外壁形成过程中的孢粉素生物合成。此外，AMD1直接与绒毡层退化延缓（TDR）相互作用，TDR是一种参与调控绒毡层降解和外壁形成的关键TF。综上所述，我们证明，AMD 1是一个重要的调节成分参与TDR介导的调节途径，以调节孢粉素的生物合成，绒毡层降解，花粉发育和外壁形成。本研究为水稻有性生殖调控网络的研究提供了新的思路，也为杂交水稻雄性不育系的基因工程育种提供了有用的靶点。
Pollen development includes a series of biological events that require precise gene regulation. Although several transcription factors (TFs) have been shown to play roles in maintaining pollen fertility, the major regulatory networks underlying tapetum development and pollen wall formation are largely unknown. Herein, we report that ABERRANT MICROSPORE DEVELOPMENT1 (AMD1), a protein annotated previously as unknown protein, is required for tapetum development and pollen exine patterning in rice (Oryza sativa L.). AMD1 encodes a grass-specific protein exhibiting transactivation activity in the nucleus and is spatiotemporally expressed in the tapetum and microspores during pollen development. Further biochemical assays indicate that AMD1 directly activates the transcription of DEFECTIVE POLLEN WALL (DPW) and POLYKETIDE SYNTHASE2 (OsPKS2), which are both implicated in sporopollenin biosynthesis during exine formation. Additionally, AMD1 directly interacts with TAPETUM DEGENERATION RETARDATION (TDR), a key TF involved in the regulation of tapetum degradation and exine formation. Taken together, we demonstrate that AMD1 is an important regulatory component involved in the TDR-mediated regulatory pathway to regulate sporopollenin biosynthesis, tapetum degradation, and exine formation for pollen development. Our work provides insights into the regulatory network of rice sexual reproduction and a useful target for genetic engineering of new male-sterile lines for hybrid rice breeding.