Bactericidal effects of high intensity focused ultrasound on Bacillus Calmette-Guerin in vivo and in vitro
Bactericidal effects of high intensity focused ultrasound on Bacillus Calmette-Guerin in vivo and in vitro
复制标题
高强度聚焦超声对卡介苗体内外杀菌效果
DOI：
10.1080/02656736.2019.1649474
复制
发表时间：
2019
影响因子：
3.1
通讯作者：
Du Yonghong
中科院分区：
文献类型：
--
作者：
Xie Shuang;Cao Hua;Li Jianhu;Adhikari Vishnu Prasad;Yang Min;Dong Yu;Li Dairong;Du Yonghong
目的：研究高强度聚焦超声（HIFU）对卡介苗（BCG）的体外和体内杀菌作用，并探讨其机制.材料和方法：以1 MHz的固定频率，对BCG培养液和大鼠皮下接种的BCG进行HIFU照射.结果：HIFU照射可显著降低细菌存活率，并引起体外和体内温度升高.结论：HIFU对卡介苗的体外和体内杀菌作用是有效的.此外，BCG悬浮液在3185和6369 W/cm 2照射15 s后，细胞壁损伤增加，导致形态学变化。此外，我们观察了在6369 W/cm 2的HIFU消融后大鼠皮下脓肿的组织学变化。感染病灶的H&E染色显示凝固性坏死，中央核溶解，炎性细胞浸润增加，以及周围核固缩和核碎裂。结论：高强度聚焦超声对大鼠卡介苗感染组织具有治疗潜力。我们的理论是，机械，空化和热效应的组合最有效地通过HIFU消灭BCG细菌。本研究有望为结核病的无创有效治疗提供生物学合理依据。
Purpose:The objective of this study was to investigate the bactericidal effects of high intensity focused ultrasound (HIFU) on Bacillus Calmette–Guerin (BCG, a substitute forMycobacterium tuberculosis)in vitroandin vivo,and to explore the underlying mechanisms.Materials and methods:HIFU, at a fixed frequency of 1 MHz, was applied to both BCG culture suspensions and subcutaneous BCG abscesses in rats.Results:HIFU irradiation significantly reduced the bacterial survival rate and caused temperature elevations bothin vitroandin vivo. Furthermore, BCG suspensions irradiated for 15 s at 3185 and 6369 W/cm2had increased cell wall damage, which resulted in morphological changes compared to the untreated control group. Additionally, we observed histological changes in the rat subcutaneous abscesses after HIFU ablation at 6369 W/cm2. H&E staining of infected lesions showed coagulative necrosis with central nucleus dissolution and increased infiltration of inflammatory cells, as well as nuclear pyknosis and nuclear fragmentation in the periphery. The volumes of the subcutaneous abscesses in the HIFU-treated group were significantly lower than those in the sham-treated group.Conclusion:HIFU has the therapeutic potential to treat BCG-infected tissues in rats. We theorize that a combination of mechanical, cavitation, and thermal effects most efficiently inactivate BCG bacteria via HIFU. This study is expected to provide a bio-plausible basis for a noninvasive and effective treatment for tuberculosis.