Single-Cell RNA Sequencing to Disentangle the Blood System.
Single-Cell RNA Sequencing to Disentangle the Blood System.
复制标题
单细胞RNA测序解开血液系统。
DOI：
10.1161/atvbaha.120.314654
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
van Galen P
中科院分区：
文献类型：
--
作者：
Acosta J;Ssozi D;van Galen P
血液系统通常被表示为具有干细胞的树状结构，干细胞通过一系列划分的步骤产生成熟的血细胞类型。虽然这种代表性几十年来一直作为分层组织组织的模型，但单细胞技术正在揭示细胞类型中间体的丰度和确保分化细胞平衡补充的分子机制。在这篇简短的评论中，我们将介绍单细胞RNA测序产生的血细胞分化的新见解，总结应用这项技术的考虑因素，并强调在单细胞分辨率下理解造血的创新。
The blood system is often represented as a tree-like structure with stem cells that give rise to mature blood cell types through a series of demarcated steps. Although this representation has served as a model of hierarchical tissue organization for decades, single-cell technologies are shedding new light on the abundance of cell type intermediates and the molecular mechanisms that ensure balanced replenishment of differentiated cells. In this Brief Review, we exemplify new insights into blood cell differentiation generated by single-cell RNA sequencing, summarize considerations for the application of this technology, and highlight innovations that are leading the way to understand hematopoiesis at the resolution of single cells.