Mass spectral compatibility of four proteomics stains
Mass spectral compatibility of four proteomics stains
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DOI：
10.1021/pr070398z
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发表时间：
2007-11-01
影响因子：
4.4
通讯作者：
Karuso, Peter
中科院分区：
文献类型：
--
作者：
Ball, Malcolm S.;Karuso, Peter
随着最近蛋白质组学市场引入新的荧光染色剂，现在有更多的选择。比较SYPRO Ruby、LavaPurple、Flamingo和Krypton总蛋白染色剂的易用性、图像质量以及与肽质量指纹图谱（PMF）（MALDI-TOF）蛋白质鉴定的兼容性。所有四种染色都产生了良好的图像，但染色模式略有不同。发现SYPRO抑制含半胱氨酸和色氨酸的肽的鉴定，这降低了蛋白质鉴定。
With the recent introduction of new fluorescence stains to the proteomics market, there is now more choice available. SYPRO Ruby, LavaPurple, Flamingo, and Krypton total protein stains were compared for ease of use, image quality, and compatibility with protein identification by peptide mass fingerprinting (PMF) (MALDI-TOF). All four stains produced good images but with slightly different staining patterns. SYPRO was found to inhibit identification of cysteine and tryptophan containing peptides, which reduced protein identification.