Valorization of sugarcane bagasse for bioethanol production through simultaneous saccharification and fermentation: Optimization and kinetic studies
Valorization of sugarcane bagasse for bioethanol production through simultaneous saccharification and fermentation: Optimization and kinetic studies
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DOI：
10.1016/j.fuel.2019.116552
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发表时间：
2020-02-15
期刊：
影响因子：
7.4
通讯作者：
Kana, E. B. Gueguim
中科院分区：
文献类型：
--
作者：
Jugwanth, Yanchal;Sewsynker-Sukai, Y.;Kana, E. B. Gueguim
本研究以制糖工业生产乙醇的废渣甘蔗渣(SCB)为研究对象，对其进行了有价化处理。建立了同时糖化发酵过程的数学模型，并进行了优化。此外，利用Logistic模型和改进的Gompertz模型分别对优化条件下酿酒酵母细胞生长和乙醇形成的动力学进行了研究。采用响应面方法(RSM)进行优化，输入参数为加酶量(20~100U/g)、温度(30~50℃)和酵母效价(1~5次)。在酵母菌滴度为1倍、加酶量为100U/g、温度为39℃的最佳工艺条件下，生物乙醇的最大浓度为4.88g/L。RSM模型表明，酶负荷参数对生物乙醇产量有显著影响。细胞生长动力学的最大比生长速率(Mu(Max))为0.15h(-1)，最大细胞生物量(X-max)为2.58g/L，改进的Gompertz模型最大生物乙醇浓度(P-m)和最大生物乙醇产量(r(p，m))分别为3.12g/L和0.29g/L/h。这些发现证明了甘蔗废料有价化在SSF工艺生产生物燃料中的潜力，并为大规模操作的工艺设计提供了见解。
This study focuses on the valorization of sugarcane bagasse (SCB), which is a waste by-product from the sugar industry for ethanol production. A simultaneous saccharification and fermentation process was modelled and optimized. In addition, the kinetics of Saccharomyces cerevisiae cell growth and ethanol formation under the optimized conditions were assessed using the logistic and modified Gompertz models respectively. The response surface methodology (RSM) was used for the optimization with input parameters consisting of enzyme loading (20-100 U/g), temperature (30-50 degrees C) and yeast titre (1-5 times). A maximum bioethanol concentration of 4.88 g/L was observed under the optimal process conditions of 1 time (yeast titre), 100 U/g (enzyme loading) and 39 degrees C (temperature). The RSM model revealed that the enzyme loading parameter significantly affected bioethanol production. Kinetics of cell growth gave a maximum specific growth rate (mu(max)) of 0.15 h(-1) and a maximum cell biomass concentration (X-max) of 2.58 g/L. The modified Gompertz model showed a maximum bioethanol concentration (P-m) and maximum bioethanol production rate (r(p, m)) of 3.12 g/L and 0.29 g/L/h respectively. These findings demonstrated the potential of sugarcane waste valorization for biofuel production through SSF processes, and provided insights into process design for large scale operations.