Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization.
Assignment of the human folate transporter gene to chromosome 21q22.3 by somatic cell hybrid analysis and in situ hybridization.
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通过体细胞杂交分析和原位杂交将人类叶酸转运蛋白基因分配到染色体 21q22.3。
DOI：
10.1006/bbrc.1995.1739
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发表时间：
1995
影响因子：
3.1
通讯作者：
Ganapathy,V
中科院分区：
文献类型：
--
作者：
Yang-Feng,TL;Ma,YY;Liang,R;Prasad,PD;Leibach,FH;Ganapathy,V
我们利用人类叶酸转运蛋白cDNA和与cDNA杂交的人类基因组克隆进行叶酸转运蛋白基因的染色体定位。以cDNA为探针进行人-鼠体细胞杂交分析，发现与人类21号染色体完全分离。利用cDNA探针对人类中期染色体进行原位杂交，将叶酸转运蛋白基因定位于21号染色体远端长臂q22.3带。这条染色体的位置是通过荧光原位杂交使用基因组克隆证实。
We have used the human folate transporter cDNA and a human genomic clone hybridizing to the cDNA to perform chromosomal mapping of the folate transporter gene. Human-rodent somatic cell hybrid analysis using the cDNA as the probe revealed perfect segregation with human chromosome 21. In situ hybridization of the cDNA probe to human metaphase chromosome spreads mapped the folate transporter gene to the distal long arm of chromosome 21, band q22.3. This chromosomal location was confirmed by fluorescence in situ hybridization using the genomic clone.