ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES DURING 3-H EXPERIMENTAL MYOCARDIAL-ISCHEMIA
ACCUMULATION OF POLYMORPHONUCLEAR LEUKOCYTES DURING 3-H EXPERIMENTAL MYOCARDIAL-ISCHEMIA
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DOI：
10.1152/ajpheart.1986.251.1.h93
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发表时间：
1986-07-01
影响因子：
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通讯作者：
SCHMIDSCHONBEIN, GW
中科院分区：
文献类型：
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作者：
ENGLER, RL;DAHLGREN, MD;SCHMIDSCHONBEIN, GW
最近的证据表明，白细胞对毛细血管的机械性阻塞在心脏中的“无复流”现象中起着重要作用。白细胞在微循环中的这种截留先于它们在缺血后的炎症反应中的公认作用。这是一个基本的流变学机制，可能与缺血性损伤和再流损伤，它还没有得到阐明。为了探讨心肌缺血过程中粒细胞的积累，我们研究了111 In-标记的自体粒细胞在急性缺血心肌中的积累，在3小时的流量减少，并没有随后的一段时间的开胸犬的复流。粒细胞聚集在所有动物的缺血性内膜中，对于大多数犬，也聚集在心外膜中。再灌注后，内皮细胞中的蓄积增强。截留的白细胞可能对阻力的增加有影响，因为白细胞在内皮细胞中的区域积累与缺血性血流在3小时的缺血呈负相关。从活检的湿重和干重测量的组织含水量显示出与截留的粒细胞的数量显着正相关。这些结果表明，侧支循环是缺血心肌中白细胞早期到达的重要机制，再灌注促进粒细胞聚集。
Recent evidence indicates that mechanical obstruction of capillaries by leukocytes plays an important role in the "no-reflow" phenomenon in the heart. This entrapment of leukocytes in the microcirculation precedes their recognized role in an inflammatory reaction following ischemia. It is a fundamental rheological mechanism that may be associated with ischemic injury and reflow injury and it has not been elucidated. To explore the accumulation of granulocytes during myocardial ischemia we studied the accumulation of 111In-labeled autologous granulocytes in acutely ischemic myocardium during 3 h of flow reduction with and without a subsequent period of reflow in open-chest dogs. Granulocytes accumulated in the ischemic endocardium of all animals and, for the majority of dogs, also in the epicardium. Accumulation in the endocardium was enhanced by reperfusion. The entrapped leukocytes may have an influence on the increase in resistance, since regional accumulation of leukocytes in the endocardium inversely correlated with ischemic blood flow during 3 h of ischemia. The tissue water content measured from the wet and dry weights of biopsies showed a significant positive correlation with the number of entrapped granulocytes. These results suggest that collateral flow is an important mechanism of leukocyte arrival early in ischemic myocardium and that reperfusion enhances granulocyte accumulation.