The renal segmental distribution of claudins changes with development
The renal segmental distribution of claudins changes with development
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DOI：
10.1046/j.1523-1755.2002.00479.x
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发表时间：
2002-08-01
影响因子：
19.6
通讯作者：
González-Mariscal, L
中科院分区：
文献类型：
--
作者：
Reyes, JL;Lamas, M;González-Mariscal, L
背景哺乳动物肾单位的渗透性在出生后的成熟过程中被调节。跨上皮电阻（TER）和紧密连接（TJ）的复杂性沿着不同的管段变化，表明构成这种结构的分子发生变化。我们研究了封闭蛋白和几种封闭蛋白在新生和成年家兔离体肾小管中的差异表达。从新生和成年兔分离的肾小管进行处理，用于封闭蛋白、封闭蛋白-1和封闭蛋白-2的免疫荧光，以及封闭蛋白-1和-2的蛋白质印迹检测。在全肾冰冻切片中检测到Claudin-5。从分离的肾小管中进行claudins-1至claudins-8的RT-PCR。免疫荧光显示，occludin，claudin-1和-2存在于细胞边界的新生儿阶段的发展。紧密连接蛋白-1被检测到在较紧的部分肾单位（远端和集合管），而紧密连接蛋白-2被发现在泄漏的部分（近端）。Claudin 5在肾脏血管中被发现。PCR扩增显示新生儿肾小管中存在claudins-1至-4。在成年人中，claudins-1，-2和-4存在于近端，汉勒氏襻和收集段; claudins-3存在于近端和收集小管中，而claudins-5和-6不存在于所有小管部分。Claudin-7仅限于近端小管，而Claudin-8则存在于近端和Henle段。occludin的分布模式是从新生儿年龄。Claudin-7和Claudin-8在出生后表达上调。每个管段表达一组特殊的封闭蛋白，这可能是负责其TJ的渗透性。
Background. Permeability properties of mammalian nephron are tuned during postnatal maturation. The transepithelial electrical resistance (TER) and complexity of tight junctions (TJs) vary along the different tubular segments, suggesting that the molecules constituting this structure change. We studied the differential expression of occludin and several claudins in isolated renal tubules from newborn and adult rabbits.Methods. Isolated renal tubules from newborn and adult rabbits were processed for occludin, claudin-1 and claudin-2 immunofluorescence, and Western blot detection of claudin-1 and -2. Claudin-5 was detected in whole kidney frozen sections. RT-PCR from isolated tubules was performed for claudins-1 to -8.Results. Immunofluorescence revealed that occludin, claudin-1 and -2 were present at the cell boundaries at the neonatal stage of development. Claudin-1 was detected in the tighter segments of the nephron (distal and collecting duct), while claudin-2 was found in the leaky portions (proximal). Claudin 5 was found in the kidney vasculature. PCR amplification revealed the presence of claudins-1 to -4 in tubules of newborns. In adults, claudins-1, -2 and -4 were present in proximal, Henle's loop and collecting segments; claudin-3 was in proximal and collecting tubules, while claudins-5 and -6 were absent from all tubular portions. Claudin-7 was restricted to proximal tubules, while claudin-8 was present in proximal and Henle's segments.Conclusions. The pattern of occludin distribution is present from the neonatal age. Claudins-7 and -8 are up-regulated after birth. Each tubular segment expresses a peculiar set of claudins that might be responsible for the permeability properties of their TJs.