Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation
Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation
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DOI：
10.1080/01496395.2018.1443139
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发表时间：
2018-01-01
影响因子：
2.8
通讯作者：
Lau, Sie Yon
中科院分区：
文献类型：
--
作者：
Acquah, Caleb;Danquah, Michael K.;Lau, Sie Yon
将适配体配体引入到圆盘整体吸附剂上，代表了一种独特的策略，用于对流分离具有高特异性和选择性的靶分子，首次进行了研究。实验结果表明，该整体柱具有良好的渗透性，其渗透率为1.67 ± 0.05 × 10 ~（-14）m ~（2）（RSD=3.2%）。适体修饰的圆盘整料的适体配体密度为480 pmol/uL。适体盘-整体柱效率的色谱分析显示在室温25 +/-2 ℃下的最佳线速度为126 cm/min（约0.25mL/min）。对应于最佳线速度的理论塔板数为128.2，理论塔板高度为0.022mm。盘适配体固定的整体系统表现出良好的选择性和分离的凝血酶从非目标。
The introduction of aptameric ligands onto disk-monolithic adsorbent, representing a unique strategy for convective isolation of target molecules with high specificity and selectivity, is investigated for the first time. Experimental results showed that the disk monolith possessed a good permeability of 1.67 +/- 0.05 x 10(-14)m(2) (RSD=3.2%). The aptameric ligand density for the aptamer-modified disk monolith was 480pmol/uL. Chromatographic analysis of the aptamer disk-monolith efficiency showed an optimum linear velocity of 126cm/min (approximate to 0.25mL/min) at room temperatures 25 +/- 2 degrees C. The theoretical number of plates corresponding to the optimum linear velocity was 128.2 with an height equivalent to the theoretical plate of 0.022mm. The disk aptamer-immobilised monolithic system demonstrated good selectivity and isolation of thrombin from non-targets.