Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology.
Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology.
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适用于微流体技术的非生物污染 PDMS 表面改性策略的最新进展。
DOI：
10.1142/s2339547817300013
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Usta OB
中科院分区：
文献类型：
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作者：
Gokaltun A;Yarmush ML;Asatekin A;Usta OB
在过去的十年中，包括快速成型技术在内的微细加工工艺发展迅速，并已达到相当成熟的阶段。这些进展鼓励并使得更广泛的用户能够使用微流体装置，其应用于生物分离以及细胞和类器官培养。因此，该领域当前的一个重大挑战是控制界面处的生物分子相互作用和开发新型生物材料以满足生物医学应用的独特需求。聚二甲基硅氧烷（PDMS）是微流控器件中应用最广泛的材料之一。这种材料的普及是其低成本，简单的制造允许快速原型，高光学透明度和透气性的结果。然而，PDMS的一个主要缺点是其疏水性和表面疏水化后的快速疏水恢复。这导致蛋白质以及小的疏水分子如治疗药物的显著非特异性吸附，限制了PDMS在生物医学微流体回路中的效用。因此，在这里，我们专注于表面分子处理的最新进展，以防止PDMS表面结垢，以提高其实用性并扩大其在生物医学应用中的用例。
In the last decade microfabrication processes including rapid prototyping techniques have advanced rapidly and achieved a fairly mature stage. These advances have encouraged and enabled the use of microfluidic devices by a wider range of users with applications in biological separations and cell and organoid cultures. Accordingly, a significant current challenge in the field is controlling biomolecular interactions at interfaces and the development of novel biomaterials to satisfy the unique needs of the biomedical applications. Poly(dimethylsiloxane) (PDMS) is one of the most widely used materials in the fabrication of microfluidic devices. The popularity of this material is the result of its low cost, simple fabrication allowing rapid prototyping, high optical transparency, and gas permeability. However, a major drawback of PDMS is its hydrophobicity and fast hydrophobic recovery after surface hydrophilization. This results in significant nonspecific adsorption of proteins as well as small hydrophobic molecules such as therapeutic drugs limiting the utility of PDMS in biomedical microfluidic circuitry. Accordingly, here, we focus on recent advances in surface molecular treatments to prevent fouling of PDMS surfaces towards improving its utility and expanding its use cases in biomedical applications.